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作 者:张超[1] 罗艳梅[1] 张家骅[1] 何晶晶 靳露[1] 赵中权[1]
机构地区:[1]西南大学重庆市牧草与草食家畜重点实验室,重庆400716 [2]重庆市合川区农委,合川401520
出 处:《遗传》2011年第6期607-612,共6页Hereditas(Beijing)
基 金:重庆市科委重大专项(编号:2008AA1033); 西南大学科研基金项目(编号:swu208002)资助
摘 要:为了研究发情周期内血浆INH B(Inhibin B)、ACT A(Activin A)和FSH(Follicle stimulating hormone)浓度与山羊繁殖力之间的关系,以高繁殖力的大足黑山羊和低繁殖力的萨能奶山羊为研究对象,采用酶联免疫法(ELISA)测定血浆中INH B、ACT A和FSH浓度,比较两个山羊品种在一个发情周期内血浆中INH B、ACT A和FSH的分泌变化规律。研究结果显示:大足黑山羊和萨能奶山羊血浆中FSH的分泌与ACT A呈正相关,与INH B呈负相关,大足黑山羊在整个发情周期内血浆中的FSH平均浓度高于萨能奶山羊,INH B和ACT A平均浓度均低于萨能奶山羊。从发情症状明显至排卵前这段时间内,大足黑山羊血浆中FSH平均浓度显著高于萨能奶山羊(P〈0.05)。结果表明,发情周期内INH B、ACT A与FSH的差异可能是导致大足黑山羊和萨能奶山羊繁殖力差异的根本原因之一。推测ACT A主要作用可能是延长卵泡期,对于山羊排卵个数的影响不起主导作用。INH B可以抑制FSH的水平,从而间接的影响排卵数。To study the relationship between the concentrations of INH B(Inhibin B),ACT A(Activin A),and FSH(Fol-licle stimulating hormone) in blood plasma and fecundity,Dazu black goat with high productivity and Sannen dairy goat with low productivity were used as experiment objects in this research.The concentrations of INH B,ACT A,and FSH in blood plasma were measured by enzyme-linked immunosorbent assay(ELISA) in order to study the secretion rule of INH B,ACT A,and FSH during an estrus cycle of two goat breeds.The results indicated that the secretion of FSH showed a positive correlation with ACT A and a negative correlation with INH B.The mean concentration of FSH in Dazu black goat was higher than that in Sanen dairy goat during a estrous cycle.However,during the time from obviously estrus to ovula-tion,the mean concentration of FSH in Dazu black goat was significantly higher than that in Sannen dairy goat HEREDITAS(Beijing) (0.01P0.05).These results showed that during an estrus cycle,the differences in INH B,ACT A,and FSH might be the reason for fecundity differences.Activin A might not be responsible for the number of eggs ovulated of goats.The main effect of ACT A may be extention of follicular stage.Inhibin B indirectly influences ovulation by regulation of FSH level.
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