食蟹猴心血管病风险因素与心血管病相关基因在外周血白细胞表达的相关性  

Correlation between cardiovascular risk factors and the expression of cardiovascular disease related genes in cynomolgus monkeys

作　　者：夏机良[1] 郝香芬[1] 曾小明 靳丽莎[1] 周彤 李学家[1] 孙云霄[1] 饶军华[1] 刘晓明[1] 彭白露[1] 

机构地区：[1]广东省昆虫研究所,广东广州510260 [2]广东蓝岛生物技术有限公司,广东广州510555

出　　处：《Zoological Research》2011年第3期293-299,共7页动物学研究（英文）

基　　金：国家科技重大专项人类重大疾病灵长类动物模型资源平台的建设项目(2011ZX09307-303-03);广州市科技计划项目大型实验动物国际认证与外包服务项目(2009A1-E051)

摘　　要：研究心血管病风险因素和心血管病相关基因表达的相关性有助于心血管病风险预警和早期诊断研究。该文采用温和的动脉粥样硬化膳食(0.053mg胆固醇/千焦、40%的能量来源于脂肪)喂养中老年雄性食蟹猴(Macaca fascicularis)(12个月),根据传统心血管病风险因素筛选低、高风险食蟹猴,然后采用荧光定量PCR技术检测113个心血管病相关基因在正常组、低风险和高风险组食蟹猴外周血白细胞内的表达差异。结果在食蟹猴外周血白细胞中共检测到65个心血管病相关基因,其中低、高风险组有16个基因相对于正常组表达上调(P<0.05),19个基因表达下调(P<0.05),另外,还有15个基因表达模式特异(P<0.05)。此外,还检测到42个心血管病相关基因在人和食蟹猴外周血白细胞内均有效表达,其中22个基因在两者之间表达模式一致。上述结果为进一步研究心血管病风险预警和早期诊断指标,缩小了基因遴选范围。The correlation between cardiovascular risk factors and cardiovascular disease related genes plays an important role in early-warning risk and early diagnosis.Thirty middle-aged male crab-eating macaques were fed a moderately atherogenic diet（0.053 mg cholesterol/kJ and 40% of calories as saturated fat） for twelve months.According to cardiovascular risk factors,we selected low-risk and high-risk crab-eating macaques,then analyzed the expression of 113 cardiovascular related genes by real-time PCR.A total of 65 genes were detected in peripheral blood leukocytes by real-time PCR.Sixteen up-regulated genes and nineteen down-regulated genes were detected in low-risk and high-risk crab-eating macaques compared to normal crab-eating macaques（P0.05）,in addition to fifteen genes that showed unique expression patterns（P0.05）.We also detected 42 genes in human peripheral blood leukocytes.The expression patterns of 22 genes were consistent between human and crab-eating macaques.These results narrowed the scope of genes for further research.

关 键 词：心血管病 风险预警 早期诊断 食蟹猴 荧光定量PCR 外周血白细胞 

分 类 号：S858.91[农业科学—临床兽医学]