中老年食蟹猴群中糖尿病相关基因的表达状态  

Expression status of diabetes-associated genes in middle and aged cynomolgus monkeys

作　　者：张秀娟[1] 李学家[1] 夏机良[1] 颜孙兴 季芳[1] 张艳春[1] 刘晓明[1,2] 彭白露[1,2] 

机构地区：[1]广东省昆虫研究所,广东广州510260 [2]广东蓝岛生物技术有限公司,广东广州510555

出　　处：《Zoological Research》2011年第3期300-306,共7页动物学研究（英文）

基　　金：国家"十二五"科技支撑计划研究项目(2011ZX093073-024);广州市重大科技专项项目(2010U1-E00811);广东省自然科学基金项目(10451026001004520)

摘　　要：该研究选择10岁以上健康中老年食蟹猴138只,按空腹血糖值(FPG)将其分为低血糖组、血糖正常组和高血糖组。采用全自动血液生化仪分别检测各组血清中总胆固醇(TCHO)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)的水平变化;采用荧光定量PCR分析食蟹猴外周血白细胞中37个糖尿病相关基因的mRNA表达情况。结果表明,血清血脂水平HDL-C、LDL-C、TCHO和TG都与FPG分组无显著相关性(P>0.05),而外周血白细胞中ACE、ACLY、PRKCB1、SLC2A4、SNAP23、VAPA、IGF2BP2、IFNG8个基因的mRNA表达水平随FPG的升高而显著上调(P<0.05)。A total of 138 middle and aged cynomolgus monkeys（Macaca fascicularis）（above 10 years） were classified into three groups based on fasting plasma glucose（FPG） values,specifically low FPG,normal FPG,and high FPG group.Total cholesterol（TCHO）,triglycerides（TG）,high density lipoprotein cholesterol（HDL-C）,and low density lipoprotein cholesterol（LDL-C） were detected in blood by automatic biochemical analyzer.The mRNA expressions of 37 diabetes-associated genes were analyzed with Real-time PCR in monocytes isolated from monkey peripheral blood.No significant correlation between the four serum lipid indictors HDL-C,LDL-C,TCHO,TG and FPG（P0.05） were found.However,the expressions of ACE,ACLY,PRKCB1,SLC2A4,SNAP23,VAPA,IGF2BP2,and IFNG were significantly enhanced when FPG increased（P0.05）.

关 键 词：食蟹猴 血脂 外周血白细胞 糖尿病 基因表达 

分 类 号：R587.1[医药卫生—内分泌] Q959.848[医药卫生—内科学]