圆叶牵牛毛状根的诱导及培养  

Induction and Culture of Hairy Roots of Pharbitis purpurea(L.)Voigt

作　　者：李丽英[1] 杨世海[1] 徐大卫[2] 张悦[1] 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118 [2]中国药材公司,北京100195

出　　处：《中国现代中药》2011年第6期30-33,共4页Modern Chinese Medicine

摘　　要：目的:利用发根农杆菌,R1601,1000,1025,15834诱导药用植物圆叶牵牛产生毛状根。方法:采用共培养方法,通过对外植体、菌种、浸染时间、预培养、共培养、外源激素等因素对转化率的影响。结果:利用发根农杆菌1601,预培养60h的叶片、叶柄为转化材料,感染6～8min,加入1mg.L-1NAA转化率最高。结论:圆叶牵牛毛状根的诱导成功,为其遗传转化提供了依据。Objective： Hairy root of Pharbitis purpurea （ L. ） Voigt were induced by the inlectlon ot tour Agrobacterium rhizogenes strains 1000,1025,15834 and R1601, and an in vitro culture system of the hairy roots was established. Methods：Hairy roots were induced by co-culture. Effects of explants,Agrobacterium rhizogenes, infecting time, pre-cuhure time , co-cuhure time and phytohormones on the transformation frequency were studied. Results： The highest induction frequency was obtained from blade leafs and leaf stalk with 60 h pre-culture which were induced by R1601 for 6 - 8 mins. The transformation frequency could be raised by culture media with 1 mg/L NAA. Conclusion： The successful induction of Pharbitis purpurea （ L. ） Voigt hairy roots provided a basis for genetic transformation.

关 键 词：圆叶牵牛 发根农杆菌 毛状根 

分 类 号：R644[医药卫生—外科学]