不同剂量的黄芪多糖对糖尿病大鼠胰腺Kir6．2表达的干预研究  

Expression of pancreatic Kir6.2 in diabetes rats and the intervention of astragalus polysaccharide at different doses

作　　者：吴葆莹[1] 吴崇旭[2] 薛承锐[1] 

机构地区：[1]天津医科大学总医院中西医结合外科,天津300052 [2]天津医科大学总医院急诊科,天津300052

出　　处：《国际外科学杂志》2011年第6期376-379,F0003,共5页International Journal of Surgery

摘　　要：目的观察不同剂量的黄芪多糖对糖尿病大鼠胰腺Kir6．2表达的干预作用。方法Wistar大鼠建立糖尿病模型后，随机分为模型组、对照组、低剂量干预组、高剂量干预组，进行糖耐量实验，检测胰腺Kit6．2mRNA和蛋白表达变化。结果模型组胰腺Kir6．2表达显著下降，两干预组Kir6．2的mRNA和蛋白表达水平较模型组有不同程度上调（P〈0．05）。结论黄芪多糖对糖尿病大鼠胰腺Kir6．2表达有一定的上调作用，可能是其发挥降糖作用的机制之一。Objective To investigate the influence of astragalus polysaccharide administration on the the expression of pancreatic Kir6.2 in diabetes rats. Methods Wistar rats were induced diabetes mellitus, then divided into model group, control group, low dose group, and high dose group randomly. After OGTT of them were observed, determination of the Kit6.2 mRNA and protein expression levels in pancreas was performed by RT-PCR and Western blotting. Results The expression of Kit6.2 in model group decreased significantly, and the expressions of Kit6.2 in low dose and high dose groups were higher than the model group （ P 〈 0.05）. Conclusion Astragalus polysaccharide displayed up- regulation to the expression of pancreatic Kir6.2, which may be a mechanism on reducing blood glucose.

关 键 词：糖尿病 KIR6.2 黄芪多糖 表达 

分 类 号：R285.5[医药卫生—中药学]