HIF-1α、EphA2表达对食管鳞癌细胞体外三维培养的影响  

Effect of expression of HIF-1α and EphA2 on vasculogenic mimicry in carcinoma

作　　者：王频[1] 王璐[1] 丁宗励[1] 曾楷峰[1] 金海林[1] 朱宏[1] 施瑞华[1] 

机构地区：[1]南京医科大学第一附属医院消化科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2011年第6期783-788,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助项目(30770991;30800511)

摘　　要：目的:探讨肿瘤细胞缺氧诱导因子1α(HIF-1α)、上皮细胞激酶(EphA2)对食管鳞癌血管生成拟态(vasculogenic mimicry,VM)的影响及两者之间的关系。方法:HIF-1α、EphA2干扰质粒分别转染食管癌Eca109细胞和TE13细胞;RT-PCR、Western blot分别检测转染细胞中HIF-1α、EphA2的抑制效率;体外三维培养模拟血管生成拟态模型观察HIF-1α、EphA2抑制后细胞管状结构数量变化。结果:HIF-1α表达抑制伴随有EphA2表达下调,而EphA2表达抑制对HIF-1α表达无明显影响。HIF-1α、EphA2 miRNA干扰质粒能有效抑制Eca109和TE13中HIF-1α、EphA2的mRNA表达,无论HIF-1α或EphA2表达抑制,均能导致三维培养中管状结构数量下降。但HIF-1α抑制对管状结构的影响要强于EphA2。结论:HIF-1α、EphA2与食管癌血管生成拟态密切相关。EphA2表达调控可能是HIF-1α参与肿瘤细胞VM的形成机制之一,此外还可能存在其他调控途径。Objective： To investigate the role and the correlation of HIF-1α and EphA2 in the process of vasculogenic mimicry inesophageal squamous cell carcinoma. Methods： Two esophageal cancer cell lines Eca109 and TE13 were transfected by plasmid har-boring interfering RNA targeting HIF-1α and EphA2. The relationship of the expression of HIF-1α and EphA2 was measured by RT-PCR and Western blot. The formation of tubular networks of Eca109 and TE13 cells was analyzed by three-dimension culture in vitro.Results： ①The expression of EphA2 in Eca109 and TE13 was inhibited with silencing HIF-1α. On the contrary, the expression ofHIF-1α in Eca109 and TE13 was not obviously inhibited with silencing EphA2. ②The mRNA expression of HIF-1α and EphA2 weremarkedly inhibited in Eca109 and TE13 lines with silencing HIF-1α and EphA2 gene. ③The numbers of tubular networks were bothremarkably decreased in Eca109 and TE13 with silencing HIF-1α and EphA2 gene, but the number of the former was more than thelatter. Conclusion： HIF-1α and EphA2 may both participate the formation of vasculogenic mimicry of esophageal squamous cell car-cinoma. EphA2 may be one of the important factors regulated by HIF-1α in the process.

关 键 词：食管鳞癌 肿瘤细胞缺氧诱导因子1α 上皮细胞激酶 血管生成拟态 RNA干扰 

分 类 号：Q813[生物学—生物工程]