瓣膜病房颤患者右心耳组织中5-羟色胺转运体的表达及临床意义  

The expression and clinical significance of 5-HTT in right atrial appendages of atrial fibrillation patients with valvular heart disease

作　　者：芮俊[1] 曹海龙[1] 王健[1] 吴延虎[1] 陈亦江[1] 

机构地区：[1]南京医科大学第一附属医院胸心外科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2011年第6期838-842,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省普通高校研究生科研创新计划(JX22013082)

摘　　要：目的:探讨瓣膜病房颤患者右心耳组织中5-羟色胺转运体(serotonin transporter,5-HTT)的表达及其临床意义。方法:将行瓣膜置换手术的患者28例,分为窦性心律(SR)组和房颤(AF)组。术中取右心耳组织,采用实时荧光定量PCR,West-ern blot及免疫组织化学法检测组织中5-HTT的表达。结果:AF组5-HTT mRNA表达水平是SR组的(2.20±1.75)倍(P<0.01);Western blot测定的AF组5-HTT蛋白水平是SR组的(2.13±0.46)倍(P﹤0.05);免疫组织化学法测定AF组光密度明显高于SR组。结论:5-HTT可能在瓣膜病房颤的发生和发展中发挥一定的作用。Objective：To investigate the expression of serotonin transporter（5-HTT）in right atrial appendages（RAAs）of patients with valvular atrial fibrillation（AF）and study its clinical significance.Methods：RAAs were obtained from 28 patients undergoing heart valve replacement.Among them,13 patients were in sinus rhythm（SR）,15 patients had AF.We compared the differential mRNA and protein expression of 5-HTT in the RAA tissues between the two groups by real-time quantitative PCR,Western blot and immunohistochemisty.Results：The mRNA expression of 5-HTT in the AF group was 2.2 times higher than that of the SR group（P﹤ 0.01）.The result of Western blot showed that the 5-HTT protein expression in AF was 2.13 times higher than that in SR（P﹤0.05）,which was confirmed by immunohistochemistry.Conclusion：These data suggested that the abnormal levels of 5-HTT in RAAs may be involved in the occurrence and maintenance of valvular AF.

关 键 词：房颤 右心耳 5-羟色胺转运体 临床意义 

分 类 号：Q786[生物学—分子生物学]