嗜碱性木素降解菌降解能力的初步研究  

Degradation Ability of Alkaliphilic Ligninolytic Bacteria

作　　者：管筱武[1] 张甲耀[1] 郑连爽[1] 罗宇煊[1] 熊鹰 

机构地区：[1]武汉大学环境科学系,武汉430072

出　　处：《中国造纸》1999年第6期19-21,共3页China Pulp & Paper

摘　　要：通过比较不同嗜碱细菌对麦草中木素、纤维素和半纤维素的降解率， 并比较不同菌株的产酶及酶活情况， 筛选出了木素降解能力较强， 而纤维素和半纤维素降解能力相对较弱的嗜碱性木素降解菌。在ｐＨ≈１０－４的条件下培养８ｄ 后， 菌株６ 降解了３２－３７％ 的麦草木素， 而纤维素和半纤维素分别降解了２１－４８％ 和２２－６９％ 。这与其产酶情况基本一致， 该菌株的酶活最高， 分别为ＭｎＰ２７１－３０Ｕ／Ｌ和ＬｉＰ４１－９４Ｕ／Ｌ。The degradation rate of lignin,cellulose and hemicellulose of wheat straw degraded by various alkaliphilic bacteria was studied.And their ligninolytic activities were compared.The selected strain had higher lignin biodegradation ability and ligninolytic activities,and lower cellulose and hemicellulose.After eight days cultivation with alkaline liquid cultivation conditions (pH 10 2),the strain degraded 32 37% klasson lignin of wheat straw,21 48% cellulose and 22 69% hemicellulose,and produced two ligninolytic enzymes:manganes dependent perosidase and lignin peroxidase,their activities were 271 30U/L and 41 94U/L respectively.

关 键 词：嗜碱性细菌 木素降解菌 生物降解能力 

分 类 号：TS71[轻工技术与工程—制浆造纸工程] TQ353.1[化学工程]