棉花纤维次生壁加厚期基因的表达谱分析  

Gene Expression Profile Analyses of Cotton Fiber During Secondary Cell Wall Deposition

作　　者：计志斌[1,2] 商海红[2] 闫恒超[1,2] 王少干[1,2] 李俊文[2] 刘爱英[2] 石玉真[2] 龚举武[2] 巩万奎[2] 王涛[2] 袁有禄[2] 

机构地区：[1]华中农业大学生命科学技术学院,武汉430072 [2]中国农业科学院棉花研究所,农业部棉花遗传改良重点实验室,安阳455000

出　　处：《分子植物育种》2011年第3期327-335,共9页Molecular Plant Breeding

基　　金：国家重点基础研究发展计划(2010CB126006);国家自然科学基金(31000732);中央级公益性科研院所基本科研业务专项(SJB1009)资助

摘　　要：本研究以高纤维强度材料0-153和转基因抗虫棉sGK9708为亲本构建的高代重组自交系群体(F6:8)中纤维强度具有显著差异的两个系(高强材料69307和低强材料69362)作为材料,利用cDNA芯片技术,对纤维次生壁加厚阶段(15DPA和20DPA)的基因表达谱进行研究。共检测到差异表达基因3383个,占cDNA芯片基因序列的11.59%;并从中选择4个代表性基因利用荧光定量PCR进行验证,其表达模式与芯片数据一致,证明芯片结果可靠。通过次生壁加厚期标志基因的表达模式研究发现,两个材料纤维发育过程不同步,高强纤维材料比低强纤维材料晚进入次生壁加厚期。利用Blast2GO软件对差异表达基因进行富集分析表明,在15DPA到20DPA,寡糖或多糖等碳水化合物、类苯基丙烷和类黄酮合成等次生代谢、氧化还原、细胞信号转导等生物学过程中相关基因出现显著性富集。而且,这些差异表达基因在两个材料中的表达模式具有时间差异性。这种表达模式的差异可能是影响棉纤维强度性状的重要原因之一。本研究为棉纤维次生壁加厚相关基因的克隆奠定了基础,同时也为深入挖掘棉纤维品质性状重要基因,并将其用于棉纤维品质性状的改良奠定了基础。In this study, two recombinant inbred lines （RILs） that differ in fiber strength, excellent （69307） and poor （69362）, was developed from an F2 population of Upland cotton （Gossypium Hirsutum L.） cross of 0-153×sGK9708. sGk9708 is the commercial transgenic variety with Bt＋CpTI genes resistant to budworm, and 0-153 with high fiber quality. Gene expression profiles of developing cotton fibers from two recombinant inbred lines （RILs） were compared using cDNA microarray which contained 29 185 uinigenes. Fiber gene expression was compared at two time points of spanning fiber transition （15 DPA） and secondary cell wall （20 DPA） biosynthesis. Approximately 3 383 genes were differentially expressed between two accessions at one or two stages studied, occupied 11.59% of the total transcripts. For independent corroboration of microarray results, 4 stage-specific genes were selected for Real-time PCR analysis of fiber gene expression. This indicated that the comparative microarray results were reliable. The different expression patterns of stage-specific development marker gene, suggest that the development processes for two materials maybe not synchronous, 69307 have longer elongation stage and delay to enter in the secondary cell wall synthesis, the contrary result for 69362. Functional classification and enrichment analysis of differentially expressed genes between the two lines revealed that the genes associated with carbohydrate metabolism （especially mono-/ploy-saccharides）, phenylpropanoid and flavonoid biosynthetic process, oxidation reduction and signal transduction have significant enrichment and temporal differences in gene expression patterns. This showed that the gene of different expression patterns may play an important role in the fiber development. The work paved the way for cloning and identifying the genes related to the secondary cell wall thickening of cotton fiber, and also set the stage for mining important genes of cotton fiber quality, and modifying cotton fiber qua

关 键 词：棉花 CDNA芯片 表达谱 纤维强度 

分 类 号：S562[农业科学—作物学]