Protective Effects of the Preconditioning with Different Doses of Sodium Aescinate on Tourniquet-induced Ischemic Reperfusion  

作　　者：杨光 崔苏扬 黄礼兵 

机构地区：[1]Department of Anesthesiology,Affiliated Hospital of Nanjing University of Traditional Chinese Medicine

出　　处：《Chinese Journal of Integrative Medicine》2011年第7期542-545,共4页中国结合医学杂志（英文版）

摘　　要：Objective：To evaluate the protective effects of sodium aescinate（SA）preconditioning on the tourniquet-induced ischemia-reperfusion（l/R）injury after limbs operation.Methods：Seventy-five patients with gradeⅠ-Ⅱissued by American Society of Anesthesiology undergoing lower limb operation were randomly assigned to 3 groups：the control group,low-dose SA-treated group and high-dose SA-treated group;each group enrolled 25 patients.The patients were treated with 5 mg and 10 mg SA 30 min before tourniquet inflation in the two treatment groups separately,while the patients in the control group received normal saline.Venous blood samples were obtained before tourniquet was inflated（T0 baseline）.And 5（T1）,10（T2）,20（T3）min after tourniquet was released.The nitric oxide（NO）,malondialdehyde（MDA）and superoxide dismutase（SOD）levels were determined by commercial kits.Meanwhile,arterial pressure（MAP）and heart rate（HR）were monitored from an automatic invigilator.Results：In the control group,MDA and NO levels were increased,and SOD and MAP were decreased significantly after tourniquet deflation compared to TO baseline（P0.05）.After tourniquet deflation,MDA and NO levels in the two treated groups were significantly decreased;meanwhile,SOD levels and MAP were increased,and the variations of HR were more stable compared with the control group（all P0.05）.There was no significant difference in all of the above between the two treated groups（P0.05）. Conclusion：The protective effects of SA preconditioning on tourniquet-induced limb l/R injury might possibly contribute to the increasing of SOD levels,and MAP and the decreasing of MDA and NO levels.Objective：To evaluate the protective effects of sodium aescinate（SA）preconditioning on the tourniquet-induced ischemia-reperfusion（l/R）injury after limbs operation.Methods：Seventy-five patients with gradeⅠ-Ⅱissued by American Society of Anesthesiology undergoing lower limb operation were randomly assigned to 3 groups：the control group,low-dose SA-treated group and high-dose SA-treated group;each group enrolled 25 patients.The patients were treated with 5 mg and 10 mg SA 30 min before tourniquet inflation in the two treatment groups separately,while the patients in the control group received normal saline.Venous blood samples were obtained before tourniquet was inflated（T0 baseline）.And 5（T1）,10（T2）,20（T3）min after tourniquet was released.The nitric oxide（NO）,malondialdehyde（MDA）and superoxide dismutase（SOD）levels were determined by commercial kits.Meanwhile,arterial pressure（MAP）and heart rate（HR）were monitored from an automatic invigilator.Results：In the control group,MDA and NO levels were increased,and SOD and MAP were decreased significantly after tourniquet deflation compared to TO baseline（P0.05）.After tourniquet deflation,MDA and NO levels in the two treated groups were significantly decreased;meanwhile,SOD levels and MAP were increased,and the variations of HR were more stable compared with the control group（all P0.05）.There was no significant difference in all of the above between the two treated groups（P0.05）. Conclusion：The protective effects of SA preconditioning on tourniquet-induced limb l/R injury might possibly contribute to the increasing of SOD levels,and MAP and the decreasing of MDA and NO levels.

关 键 词：ischemic reperfusion injury sodium aescinate LIMB 

分 类 号：R658.3[医药卫生—外科学]