热灭活白念珠菌刺激PBMC对颗粒溶素和TNF-α表达的影响  

Effect of heat-killed Candida albicans on the expression of granulysin and tumor necrosis factor-α in peripheral blood mononuclear cells

作　　者：黄元兰[1] 孙懿[1] 胡志德[1] 陈孙孝[1] 仲人前[1] 邓安梅[1] 

机构地区：[1]第二军医大学长征医院实验诊断科全军临床免疫中心,上海200003

出　　处：《中国免疫学杂志》2011年第6期483-487,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金(30770997、30772017、30972730、81072479);上海科委基金(09JC1405400、08QH14001)资助

摘　　要：目的:研究热灭活白念珠菌刺激健康个体外周血单个核细胞对颗粒溶素和TNF-α表达的影响。方法:在热灭活白念珠菌刺激的情况下,采用ELISA和RT-PCR检测颗粒溶素的表达;采用SrCl2以及siRNA下调PBMC内颗粒溶素的表达,或者加入外源性颗粒溶素,观察TNF-α在mRNA和蛋白水平表达的变化。结果:热灭活白念珠菌刺激可以诱导PBMC合成颗粒溶素和TNF-α,采用SrCl2或siRNA下调颗粒溶素的表达均会引起TNF-α在mRNA和蛋白水平表达的下调,而加入外源性的颗粒溶素可以促进TNF-α的表达。结论:热灭活白念珠菌刺激PBMC可以引起颗粒溶素和TNF-α的表达,且颗粒溶素可能是促进TNF-α表达的因素。Objective：To investigate the effect of heat-killed Candida albicans on the expression of granulysin and tumor necrosis factor-α in peripheral blood mononuclear cells of healthy ii,dividuals. Methods： EHSA and RT-PCR were used to detect the expression of granulysin during heat-killed Candida albicans stimulation. The alteration of tumor necrosis factor-or expression was detected by EHSA and RT-PCR after granulysin＇ s expression was kilocked down by siRNA or SrCI2. Results： Heat-killed Candida albicans induced the expression of granulysin and tumor necrosis factor-α in peripheral blood mononuclear cells. Tile production of tumor necrosis factor-α decreased follwing expression of granulysin was silenced by siRNA or SrC12 and increased after extrinsic granulysin was added. Conclusion： Heat-killed Candida albicans could induee the production of granulysin and tumor necrosis factor-α in peripheral blood mononuclear cells. Inaddition, granulysin could promote the heat-killed Candida albicans-triggering expression of ttunor necrosis factor-α,

关 键 词：白念珠菌 颗粒溶素 TNF-Α 

分 类 号：R392.12[医药卫生—免疫学]