胸腺素α原作为约氏疟原虫疫苗免疫佐剂的初步研究  

Study on the prothymosin α as vaccine adjuvant of P.yoelii

作　　者：高吉青[1] 刘升发[1] 韩伟[1] 张亭[1] 吴汉洲[1] 王世媛 杨彩霞[1] 洪凌仙[1] 章军[1] 周克夫[1] 

机构地区：[1]厦门大学生命科学学院细胞生物学和肿瘤细胞工程教育部重点实验室,厦门361005 [2]厦门伯赛基因转录技术有限公司,厦门361005

出　　处：《中国免疫学杂志》2011年第6期552-555,共4页Chinese Journal of Immunology

基　　金：厦门市科技项目(3502Z20083004);国家"973"项目(2007CB513103)资助

摘　　要：目的:研究胸腺素α原(Prothymosinα,ProTα)作为约氏疟原虫疫苗免疫佐剂的作用。方法:提取P.yoelii-17XNL全蛋白作为抗原,用胸腺素α原作为免疫佐剂,免疫小鼠。具体方案为:昆明小鼠分为4组,每组6只,A组免疫P.yoelii-17XNL全蛋白+ProTα;B组免疫P.yoelii-17XNL全蛋白;C组只注射ProTα;D组为空白对照,以相同体积的生理盐水代替。免疫结束后感染致死的P.yoelii-17XL,1×107个虫/只小鼠。结果:感染后的前10天A组小鼠疟原虫血症平均值要低于其他三组,且最终有3只小鼠存活下来,存活率50%,C组有一只小鼠存活,B、D组小鼠全部死亡。结论:用P.yoelii-17XNL全蛋白做抗原,用ProTα作为佐剂,比单独用P.yoelii-17XNL全蛋白对小鼠有更好的免疫保护作用,提示了ProTα可以成为一种有潜力的蛋白疫苗。Objective：To investigate the function of prothymosin α（ProTα） as vaccine adjuvant of P. yoelii. Methods： The mice were immunized with the total protein extracted from P. yoelii-17XNL as antigen, together with prothyinosin α as adjuvant. Programs： Kurnming mice were divided into A, B, C and D group. A group was immunized with P. yoelii-17XNL total protein and ProTa; B group was immunized with P. yoelii-17XNL; C group was only injected with ProTα; D group was the control, only injected with physiological saline. And then, the mice of each group was infected with P. yoelii-17XL, the dosage was 1 × 107/mice. Results： The pmrasitemia of A group-mice was lower than the other three groups in the first 10 days after infection, and eventually there were three mice survived in A group, the survival rate was 50%, one mouse survived in C group, all of mice in B group and D group died. Conclusion： Mice immunized with P. yoelii-17XNL total protein as antigen together with ProTα as adjuvants, had better immune protection than those immunized with P. yoelii-17XNL protein only. The present results suggest that the ProTα can act as a potential adjuvant in protein vaccine.

关 键 词：约氏疟原虫 疫苗 胸腺素Α原 免疫佐剂 

分 类 号：R12-33[医药卫生—环境卫生学]