不同基因型大豆Fd-GOGAT基因cDNA序列的克隆与分析  

Cloning and Analysis of Fd-GOGAT Genes from Different Soybean Varieties

作　　者：陈丽华 刘丽君[2] 刘页丽[2] 祖伟[2] 

机构地区：[1]国家农业标准化监测与研究中心,黑龙江哈尔滨150036 [2]东北农业大学农学院,黑龙江哈尔滨150030

出　　处：《大豆科学》2011年第3期374-378,共5页Soybean Science

基　　金：国家"十一五"科技攻关资助项目(2006BAD21B01)

摘　　要：克隆了6个不同基因型大豆的Fd-GOGAT基因,序列比对分析结果表明:不同基因型大豆的Fd-GOGAT基因序列相似性很高,可设计出通用Real-time PCR引物,检测Fd-GOGAT基因的表达规律。在东农42 Fd-GOGAT基因序列的编码区内出现了1个8核苷酸的缺失,产生了移码突变,导致其C端缺失了79个氨基酸的保守序列,造成谷氨酸合成酶大亚基C端保守结构域(gltB_C)不完整。Fd-GOGAT基因分子进化树和蛋白质序列分子进化树都显示东农42、半野生大豆、东农46 Fd-GOGAT基因序列进化距离较近。Fd-GOGAT genes from six different soybean varieties were cloned.The comparative analysis showed the sequences of GOGAT genes in different soybean varieties were very identical,so we can design general real-time PCR primer to detect express regulation of Fd-GOGAT genes.The coding sequence of Fd-GOGAT gene in Dongnong42 appeared an absence of 8 nucleotides,which brought frameshift mutation,led to its C-terminal lack of 79 amimo acids conserved sequence and the GS1 big subunit C-terminal conserved domain（gltB_C）were not in their integrity.Molecular and protein evolution tree analysis showed Dongnong 42,Dongnong 46 and G.gracilis had similar evolution distance for Fd-GOGAT gene.

关 键 词：大豆 Fd-GOGAT基因 克隆 

分 类 号：S565.1[农业科学—作物学]