农杆菌介导植酸酶基因转化大豆的研究  

Transformation of Phytase Gene into Soybean via Agrobacterium-mediated Method

作　　者：刘琦[1] 夏善勇[1] 张军[2] 段忠卫[2] 刘鑫磊[3] 李希臣[1] 

机构地区：[1]黑龙江省农业科学院生物技术研究所,黑龙江哈尔滨150086 [2]东北农业大学生命科学学院,黑龙江哈尔滨150086 [3]黑龙江省农业科学院大豆研究所,黑龙江哈尔滨150086

出　　处：《大豆科学》2011年第3期397-400,共4页Soybean Science

基　　金：国家转基因专项资助项目(ZJN04-0402);黑龙江省农业科学院创新工程资助项目

摘　　要：以大豆胚尖为受体,利用农杆菌介导法将来源于泡盛曲霉的植酸酶基因phy转入黑农37和吉林小粒豆中。此法取材方便,操作简单,产生嵌合体的可能性低。在筛选培养基中经草胺膦(1.0 mg.L-1)筛选,获得抗性植株。对获得的草胺磷抗性植株采用除草剂(10%Basta)筛查表型鉴定与目的基因、筛选标记基因PCR分子检测相结合的方法,检测结果直观、可信度高。最终获得5株阳性植株,转化率为0.5%。The Phy gene cloned from Aspergillus awamori was transformed into soybean varieties Heinong37 and Jilinxiaoli soybean through Agrobacterium-mediated method with soybean embryonic tips as acceptors.The method has advantage of easy operation and lower chimera rate.The regenerated plants were screened by glufosinate-ammonium（1.0 mg·L^-1）of medium.The regenerated plantlets resistant to glufosinate-ammonium were detected by combining the PCR analysis of the target gene as well as selective marker gene and the herbicide screening.The result was intuitive and credible.Finally,5 positive plants were obtained,and the transformation efficiency reached 0.5%.

关 键 词：大豆胚尖 农杆菌 植酸酶基因 

分 类 号：S565.1[农业科学—作物学]