参芎葡萄糖在治疗肺纤维化中的作用  

Effect of Shenxiong glucose on pulmonary fibrosis

作　　者：赵丽丽[1] 韩磊[1] 鲍文华[2] 

机构地区：[1]牡丹江医学院红旗医院呼吸内科,黑龙江牡丹江157003 [2]佳木斯大学附属第一医院呼吸内科,黑龙江佳木斯154003

出　　处：《黑龙江医药科学》2011年第3期13-14,共2页Heilongjiang Medicine and Pharmacy

摘　　要：目的:研究中药参芎葡萄糖在大鼠肺纤维化治疗中的作用。方法:①实验分组:先将健康Wistar大鼠60只随机分两组,对照组20只、模型组40只,若模型成功将模型组再分为模型组(单纯肺纤维化组)、参芎葡萄糖治疗肺纤维化组(简称参芎组),地塞米松治疗肺纤维化组(简称地塞组)。②模型制备:采用气管内注射博来霉素(BLM-A5)建立肺纤维化模型,并在此基础上第15天开始腹腔注射参芎和地塞米松制备参芎组与地塞组动物模型。③评价:分别于实验的第7天、14天将对照组、模型组大鼠随机处死5只,通过对大鼠肺组织形态学、病理学观察,评价模型是否成功,若成功进一步将模型组剩余大鼠分为模型组(单纯肺纤维化组)、参芎组、地塞组,每组各10只,分别于实验的第21、28天每组各处死5只大鼠。④观察指标:实验第7、14、21、28天各组大鼠取肺组织行HE染色和Mallory三色染色。结果:①造模后首先出现肺泡炎,随着时间的延长,炎性反应逐渐减轻,肺纤维化程度逐渐加重。②参芎组纤维化程度较地塞组明显减轻。结论:①经过观察,采用气管内注射博来霉素(BLM-A5)的方法成功的制备了大鼠肺纤维化模型。②地塞米松、参芎可以有效的拮抗肺纤维化的形成,但两者之间差异不大,因而参芎可为肺纤维化的治疗提供一种新的方法。Objective：To study the effect of Chinese medicine Shenxiong glucose on pulmonary fibrosis of rats.Methods：①Experimental groups： first of all,60 healthy Wistar rats were randomly divided into 2 groups,control group 20 and the model group 40.After the model came to success,the model group would be secondly divided into model group（simple pulmonary fibrosis group）,Shenxiong glucose treatment of pulmonary fibrosis group（referred to as Shenxiong group）,dexamethasone treatment of pulmonary fibrosis group（referred to as dexamethasone group）.②Model preparation： we used endotracheal bleomycin injection to prepare the animal models of pulmonary fibrosis,and on this basis,Shenxiong and dexamethasone were injected to prepare Shenxiong group and dexamethasone group of animal models.3 from 15th day.③Evaluation： at days 7 and 14 of the experiment respectively,5 rats were randomly killed,and by observing rat lung tissue morphology and pathology,we evaluated whether the model succeeded.If success,the remaining rats in model group would be divided into model group（simple pulmonary fibrosis group）,Shenxiong group and dexamethasone group,with 10 in each group,and 5 rats in each group can be killed at the 21th day and 28th day,respectively.④Outcome assessment： at days 7,14,21 and 28,we began HE dyeing and Mallory 3-color staining in pulmonary tissue of each group.Results：①After the model was prepared,at first,pulmonary alveolitis appeared,and with time increasing,inflammatory reaction was gradually reduced,the degree of pulmonary fibrosis was progressive.②The degree of pulmonary fibrosis was decreased significantly in Shenxiong group compared with dexamethasone group.Conclusion：①The model of pulmonary fibrosis in ratscan be successfully prepared by intratracheal bleomycin injection method（BLM-A5）.②Dexamethasone and Shenxiong can be used as effective antagonists in pulmonary fibrosis,with no a significant difference between the two,and therefore,Shenxiong can provide a new way to tre

关 键 词：参芎葡萄糖 地塞米松 肺纤维化 

分 类 号：R563[医药卫生—呼吸系统]