甘蓝型油菜BnPex16p基因cDNA的克隆与表达  

Molecular cloning and characterization of BnPex16p gene from Brassica napus

作　　者：吴学龙[1] 刘智宏[1] 何海燕[1] 郎春秀[1] 陈锦清[1] 

机构地区：[1]浙江省植物基因工程代谢重点实验室,浙江省农业科学院病毒学与生物技术研究所,浙江杭州310021

出　　处：《中国油料作物学报》2011年第3期203-209,共7页Chinese Journal of Oil Crop Sciences

基　　金：国家863计划(2006AA10A1132007AA02Z128);浙江省自然科学基金(Y306097)

摘　　要：利用模式植物拟南芥AtPex16p/SSE1基因序列与油菜数据库BBSRC BrassicaDB比对,得到甘蓝型油菜(Brassica napus)同源EST序列,拼接出油菜Pex16p基因全长cDNA电子克隆,然后设计全长引物,以甘蓝型油菜种子cDNA为模板,克隆Pex16p基因,得到全长为1 101bp的cDNA,编码366个氨基酸的蛋白,命名为BnPex16p。Bn-Pex16p基因序列与拟南芥AtPex16p/SSE1同源,其蛋白与拟南芥同源蛋白具有完全相同的PTS2型过氧化物酶体定位肽,进化关系相近。半定量PCR(RT-PCR)发现BnPex16p基因在油菜幼嫩的根、茎、叶和种子中均有高丰度表达,在种子发育过程的中期和后期表达水平增加,暗示该基因在油脂的积累中有重要作用。According to AtPexl6p/SSE1 and ESTs sequences from Brassica napus by wu -blast searching in BBSRC BrassicaDBA, a full - length cDNA encoding Pexl6p from developing seeds of Brassica napus var CY2 was cloned. The gene was designated as BnPexl6p which was 1 101bp in length encoding 366 amino acids. It was found that BnPexl6p gene was homologous to AtPex16p/SSE1. They both had a PTS2 type peroxisome targeting signal and a close evolutionary distance. Semi - quantitative RT - PCR analysis demonstrated that BnPexl6p was constitutiveiy expressed in CY2 tissues and its expression increased in seeds during the developmental stages. It indicated that BnPexl6p gene might have crucial roles in oil accumulation in B. napus.

关 键 词：过氧化物酶体 油菜Pex16p基因 序列分析 甘蓝型油菜 

分 类 号：Q785[生物学—分子生物学] S565.4[农业科学—作物学]