三叶香茶菜药材HPLC指纹图谱的研究  

HPLC Fingerprints Study on Herba Isodonis Ternifoliae/Plectranthus Ternifolius

作　　者：王勤[1] 陈勇[1] 李耀华[1] 卢森华[1] 曾海生[1] 巫繁菁[1] 韦玉燕[1] 

机构地区：[1]广西中医学院,南宁市明秀东路179号530001

出　　处：《广西中医药》2011年第3期57-59,共3页Guangxi Journal of Traditional Chinese Medicine

基　　金：广西科技厅科技攻关项目(桂科攻0537025-3B)

摘　　要：目的:建立三叶香茶菜药材的HPLC指纹图谱分析方法,科学评价并有效控制三叶香茶菜药材质量。方法:采用反相高效液相色谱法,C18柱(4.6×250mm,5μm),乙腈-甲醇-0.05%磷酸溶液梯度洗脱,流速1.0ml/min,检测波长207nm,柱温30℃。结果:建立了三叶香茶菜药材的HPLC指纹图谱,以芒果苷对照品为参照峰,确立了三叶香茶菜药材指纹图谱中的9个共有峰,12批样品的指纹图谱相似度均大于0.90,符合指纹图谱研究技术的要求。结论:所建立的三叶香茶菜药材HPLC指纹图谱,精密度、重现性和稳定性较好,有助于三叶香茶菜药材的规范化种植及药材的质量控制。Objective：To establish the method of fingerprint analysis in Herba isodonis ternifoliae（HIT）by HPLC to establish a sensitive and specific method for controlling the quality of HIT.Methods：RP-HPLC was used on a C18 column（4.6 ×250 mm,5 μm） with a mixture solution of acetonitrile,methanol and 0.05% H3PO4 as mobile phase for gradient elution.The flow was 1.0 ml/min and the column temperature was 30 ℃ while the detection wavelength was 207nm.Results：The HPLC fingerprints of HIT was established and showed 9 characteristic peaks while the mangiferin internal standard has been used as reference peak.The similarity of fingerprints in 12 batch HIT samples were higher than 0.90 and meet the requirements of fingerprints technology.Conclusion：The RP-HPLC fingerprint chromatogram of HIT which established in study is highly accuracy,good reproducibility and stability,can be the reference and useful for actualize standardization planting and the quality control of HIT.

关 键 词：三叶香茶菜 HPLC 指纹图谱 相似度 

分 类 号：R284.1[医药卫生—中药学]