γ射线诱发人外周血淋巴细胞T细胞受体基因突变剂量-效应和时间-效应关系  

Dose-effect relationship and time-effect relationship of T cell receptor gene mutation induced by γ- rays in human lymphocytes of peripheral blood

作　　者：齐雪松[1] 吕慧敏[1] 王春燕[1] 张伟[1] 郝述霞[1] 苟巧[1] 佟鹏[1] 刘青杰[1] 苏旭[1] 

机构地区：[1]中国疾病预防控制中心辐射防护与核安全医学所 ,北京100088

出　　处：《中华放射医学与防护杂志》2011年第3期286-289,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：基金项目：卫生行业科研专项（200802018）

摘　　要：目的初步建立T细胞受体（TCR）突变频率的剂量-效应和时间-效应模型，为探讨TCR作为估算辐射生物剂量计提供依据。方法将10名健康成年人的外周血淋巴细胞分成两组，第1组4人（男性）的外周血淋巴细胞分别给予0、0．5、1．0、1．5、2．0、2．5、3．0、3．5、4．0和5．0Gyγ射线照射，用于拟合剂量-效应曲线，第2组6人（男女各半）的外周血淋巴细胞给予2Gy．y射线照射，用于拟合时间-效应曲线。用流式细胞仪进行计数检测，计算TCR基因突变频率。结果1射线照射诱发TCRMF的辐射剂量-效应曲线，拟合最佳的模型为二次方程模型：TCRMF=92．14＋22．61D2（R2adj=0．65）；γ射线照射诱发TCRMF的辐射时间-效应曲线，拟合最佳的模型为二次多项式方程模型：TCRMF=3．74＋743．66T＋308．64T2（R2adj=0．79）。结论0～5Gy范围内TCR基因突变频率与辐射剂量存在剂量-效应关系。照后4d内TCR基因突变频率随时间的延长而继续增加，存在时间-效应关系。Objective To study the dose-effect relationship and tlme-effect relationship of T cell receptor （TCR） gene mutation induced by γ-rays in lymphocytes of human peripheral blood. Methods Samples of peripheral blood were collected from 10 healthy adults and lymphocytes were separated. Four samples from males used to fit time-effect curve were exposed to γ-rays at the doses of 0, 0. 5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, and 5.0 Gy, respectively, and 6 samples from 3 males and 3 females used to fit dose-effect curves were exposed toγ-rays of the dose of 2 Gy. Flow cytometry was used to detect the mutation frequency of TCR gene （TCR MF）. Radiation dose-effect curves and time-effect curves were fitted and optimal mathematical models were selected respectively. Results The optimal mathematical model for radiation dose-effect was quadratic equation model： TCR MF = 92.14 ＋ 22.61D2 （ R2adj = 0.65） adj The optimal mathematical model for radiation time-effect was quadratic polynomial equation model： TCR MF = 3.74 ＋ 743.66T ＋ 308.64T2 （ R2adj = 0. 79 ） Conclusions TCR MF is increased as the γ-ray irradiation dose increases within the range of 0 - 5 Gy, and TCR MF is increased with the lapse of time within the range of 4 days after γ-ray radiation.

关 键 词：Γ射线 T细胞受体 剂量-效应关系 时间-效应关系 生物剂量计 

分 类 号：R144[医药卫生—公共卫生与预防医学]