油桐尺蠖核型多角体病毒不同分离株的比较  

Comparison among the isolates of BusuNPV

作　　者：陈川[1] 张永安[1] 王玉珠[1] 曲良建[1] 林思诚 马沛沛 

机构地区：[1]中国林业科学研究院森林生态环境与保护研究所国家林业局森林保护学重点实验室,北京100091 [2]茂名市林业科学研究所,广东茂名525000

出　　处：《中国森林病虫》2011年第4期4-7,共4页Forest Pest and Disease

基　　金：林业公益行业专项基金(200704003)

摘　　要：该文对油桐尺蠖核型多角体病毒(Buzura suppressatia nucleopolyd rovirus,BusuNPV)的不同毒株进行了比较研究。毒株LF,SF,WF对2～3龄油桐尺蠖幼虫的LC50分别为4.39×104,6.65×104,1.02×105PIB/mL;LT50分别为5.59,5.69,5.17 d。各分离株均为单粒包埋核型多角体病毒,病毒粒子大小为(0.195～0.246)μm×(0.054～0.072)μm,多角体直径为0.85～1.50μm。对BusuNPV全基因组序列进行单酶解时,除EcoRⅠ外其它内切酶酶解条带均较少,分子量大,不易进行分离株比较。双酶解BusuNPV全基因组序列时,DNA充分被消化,条带较多,但各分离株的酶切条带数目、大小不存在差别。Different isolates of the Buzura suppressatia nucleopolydrovirus were studied. The LCs0 of LF,SF,WF tested with larvae in 2 or 3 instar of Buzura suppressatia were 4. 39× 10^4,6. 65 × 10^4, 1.02 × 10^5 PIB/mL respectively. LT50 of the above were 5.59,5.69,5.17 d. They were all single-parti- cle-embedded nuclear polyhedrosis virus and the diameters of the polyhedron bodies were 0. 85- 1.50 μm. The virus particle size was（0. 195-0. 246） μm ×（0. 054-0. 072） μm. In the single enzyme digestion of the whole genome sequence of BusuNPV, All endonuclease digestion bands except for EcoR I were small and molecular weight, which was difficult for the comprison of the isolates. When digesting the BusuNPV by double enzyme genome sequence, DNA was fully digested. However, restric- tion enzyme digestion patterns showed that the enzyme band number and size of the three isolates were not difference.

关 键 词：油桐尺蠖核型多角体病毒 生物测定 核酸酶切图谱 

分 类 号：S763.12[农业科学—森林保护学]