柴胡疏肝散对慢性应激抑郁模型小鼠行为与脑促肾上腺皮质激素释放激素基因表达的影响  

Effects of Chaihu Shugan San on behavior and CRHmRNA expression in the brain of mice with chronicmild unpredicted stress depression

作　　者：张春虎[1] 胡随瑜[1] 李云辉[1] 黄熙[1] 邓颖[1] 王杨[1] 

机构地区：[1]中南大学湘雅医院中西医结合研究所,国家中医药管理局中医肝脏象重点研究室,长沙410008

出　　处：《中华行为医学与脑科学杂志》2011年第6期485-487,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金青年基金资助项目（30801506）

摘　　要：目的研究柴胡疏肝散对慢性应激抑郁模型小鼠行为学和血浆皮质醇水平及额叶皮质、下丘脑促肾上腺皮质激素释放激素（CRH）mRNA表达的影响。方法40只雄性昆明小鼠随机分为空白对照组、模型对照组、氟西汀对照组、柴胡疏肝散组，每组n=10。采用慢性轻度不可预见性应激复制小鼠抑郁模型。所有动物于造模第8天开始灌胃，分别灌服等体积生理盐水（空白对照组、模型组）、氟西汀（2．6mg·kg＾-1）、柴胡疏肝散（8．45mg·kg＾-1）药液，连续2周。观察小鼠行为学变化，放免法检测血浆皮质醇含量，RT-PCR法检测额叶皮质及下丘脑CRHmRNA表达。结果柴胡疏肝散组体质量增加[（13．22±3．57）g]、糖水消耗量[（1．90±0．74）ml]均高于模型对照组，差异具有显著性（P〈0．05或P〈0．01）；强迫游泳不动时间[（62．20±13．73）s]、悬尾不动时间[（58．10±11．71）s]、血浆皮质醇含量[（12．61±4．63）ng／L]、额叶皮质（0．34±0．10）、下丘脑（0．70±0．09）CRHmRNA表达显著低于模型组，差异具有显著性（P〈0．05或P〈0．01）。结论柴胡疏肝散可改善慢性应激抑郁模型小鼠抑郁行为，降低血浆皮质醇含量及额叶皮质、下丘脑CRHmRNA的表达。Objective To investigate the effects of Chaihu Shugan San（CHSGS） ,a compound traditional Chinese herbal medicine, on the behavior and plasma levels of corticosterone level and cortieotrophin releasing hormone （CRH） mRNA expression in the prefrontal cortex and the hypothalamus of mice with chronic mild unpredicted stress depression. Methods 40 adult male Kunming mice were randomly divided into 4 groups： the normal control group,the model control group, the fluoxetine group and CHSGS group. Except the normal control group, mice were exposed to an unpredicted sequence of mild stressor for continuous 21 days to induce depression. Since the 8th day, mice were intragastrically administered with equal volume agents respectively for 14 days （normal saline for the normal control group and the model group ,fluoxetine（2.6 mg · kg^-l ） for fluoxetine group and CHSGS （8.45 g · kg^-l） for CHSGS group）. Body weight and behaviors were investigated, the plasma levels of CORT in different groups were detected by radioimmunoassay, and the mRNA expression in the prefrontal cortex and the hypothalamus were semiquantified by the RT-PCR method. Results In eomparision with the model group, body weight changes （ （ 13.22 ± 3.57 ） g） and consumption of sucrose solution （ （ 1.90 ± 0.74 ） ml） of CHSGS groups were significantly increased, and the duration of immobility during the forced swimming （ （62.20 ±13.73 ）s） and tail suspension （ （ 58.10 ±11.71 ） s） , the levels of plasma CORT （ （ 12.61± 4.63 ） rig/L） and the CRHmRNA expression in the prefrontal cortex（0.34 ±0.10） and the hypothalamus（0.70± 0.09） were significantly decreased. Conclusion CHSGS can reverse the depressive behavior,reduced the levels of plasma CORT and down-regulated CRHmRNA expression in the prefrontal cortex and the hypothalamus of the depressive mouse caused chronic stress.

关 键 词：柴胡疏肝散 应激 小鼠 行为 促肾上腺皮质激素释放激素 

分 类 号：R277.7[医药卫生—中医学]