丁苯酞对酒精依赖大鼠海马硫化氢含量及NMDA受体2B亚基表达的影响  

Effect of butylphthalide on H2 S content and the expression of NR2B in the hippocampus of alcohol dependence rats

作　　者：杜爱林[1] 徐春阳[1] 姜洪波[1] 邵坤[1] 陈璐[1] 侯福佳[1] 张瑞岭 

机构地区：[1]河南省生物精神病学重点实验室新乡医学院,新乡453003 [2]河南省生物精神病学重点实验室第二附属医学院,新乡453003

出　　处：《中华行为医学与脑科学杂志》2011年第6期491-493,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：基金项目：河南省生物精神病学重点实验室开放课题;河南省科技厅课题资助项目（094200510005）;河南省卫生科技创新型人才工程专项经费资助项目

摘　　要：目的研究丁苯酞（NBP）对酒精依赖大鼠海马硫化氢（H2S）含量及N-甲基-D-天冬氨酸（NMDA）受体2B亚基（NR2B）表达的影响。方法84只SD雄性大鼠随机分为6组，除正常组外，各组饮含6％（V／V）酒精水溶液28d，饮6％（V／V）酒精水溶液14d后，NBP低、中、高剂量组大鼠灌胃不同剂量NBP，检测戒断症状评分、海马组织H2S含量、胱硫醚β-合酶（CBS）活性以及NR2B的表达。结果NBP中剂量组大鼠戒断症状评分[（12．27±1．19）分]、H2S含量[（30．25±8．82）nmol／g]、CBS活性[（72．44±7．46）nmol·g^-1·h^-1]及NR2BmRNA的表达（19．47±0．86）与实验对照组大鼠戒断症状评分[（14．09±2．21）分]、H2S含量[（44．50±6．65）nmolfg]、CBS活性[（79．06±4．57）nmol·g^-1·h。]及NR2BmRNA的表达（29．13±1．39）相比均降低，差异有显著性（P〈0．05）。NBP高剂量组大鼠戒断症状评分[（12．18±1．08）分]、H2S含量[（33．00±5．38）nmol／g]、CBS活性[（67．81±9．37）nmol·g^-1·h^-1]及NR2BmRNA的表达（23．12±1．86）与实验对照组大鼠相比均降低，差异有显著性（P〈0．05）。结论NBP能够减轻酒精依赖大鼠的戒断症状，可能与NBP抑制H2S／CBS体系表达及抑制NR2BmRNA表达有关。Objective To investigate the effect of butylphthalide （NBP） on H2 S content and the expression of NR2B in the hippocampus of alcohol dependence rats. Methods A total of 84 SD male rats were randomly divided into 6 groups. Except for the normal group, other groups were subjected to alcohol solution with concentration of 6% （V/V） for 28 d. Drug intervention began at the 14th day, and rats in the low, medium, high dose group were treated with NBP with a different concentration. Erden abstinence scoring was used to evaluate the rats withdrawal symptom. H2S content was measured in one side of hippoeampus and CBS activity was tested in the other side of hippocampus. Hippocampus of 3 rats from each group was used to investigate NR2B mRNA level. Resuits Withdrawal symptom score （ 12.27 ±1.19 ）, H2 S content （ 30.25 ±8.82 ）, CBS activity （ 72.44± 7.46 ） and NR2B mRNA expression（ 19.47± 0.86） in medium dose NBP group rats were lower than withdrawal symptom score （ 14.09±2.21 ） , H2 S content （44.50±6, 65 ） , CBS activity （ 79.06 ± 4.57 ） and NR2B mRNA expression （29.13 ± 1.39） in experimental control group （P 〈 0.05 ）. Withdrawal symptom score （ 12.18± 1.08 ） , H2 S con- tent （33.00 ± 5.38 ）, CBS activity（67.81 ± 9.37 ） and NR2B mRNA expression （23.12 ±1.86 ） in high dose NBP group rats were lower than experimental control group （P 〈 0.05 ）. Conclusion NBP can reduce withdrawal symptoms of alcohol dependence rats,may be related to decreased expression of H2S/CBS system, and NR2B mRNA expression.

关 键 词：丁苯酞 酒精依赖 硫化氢 N-甲基-D-天冬氨酸受体2B亚基 

分 类 号：R965[医药卫生—药理学]