豆瓣绿(Peperomia obtusifolia)的组织培养与快速繁殖研究  

Studies on Tissue Culture and Rapid Propagation of Peperomia obtusifolia

作　　者：王涛[1,2] 彭立新[1] 

机构地区：[1]天津农学院园艺系,天津300384 [2]四川农业大学生命科学与理学院,四川雅安625014

出　　处：《天津农学院学报》2011年第2期1-5,共5页Journal of Tianjin Agricultural University

基　　金：天津市农委科技成果转化项目"耐盐植物生物改良滨海盐渍地技术示范"(0802210)

摘　　要：介绍了以豆瓣绿带芽茎段、叶片、花序为外植体的组织培养和快速繁殖研究。结果表明:可用于豆瓣绿组织培养的最适外植体为带芽茎段。诱导芽生长的最适培养基为MS+6-BA1.00 mg.L-1+NAA 1.00 mg.L-1+VC60 mg.L-1,诱导率为95%。诱导芽增殖的最适培养基为MS+6-BA 1.00 mg.L-1+NAA 0.03 mg.L-1+KT2.50 mg.L-1+GA30.05 mg.L-1,增殖倍数为10。生根培养基的最适配方为1/2 MS+NAA 0.25 mg.L-1+蔗糖2%。In this experiment, the stems with buds, leaves and inflorescence of Peperomia obtusifolia were chosen as the explants. The results show that the best explants for the tissue culture were the stems with buds. The optimal medium to induce the buds＇growth was MS ＋ 6 - BA 1.00 mg· L^-1 ＋ NAA 1.00 mg·L^-1 ＋ Vc 60 mg·L^-1, and the inducing rate was 95 %. The optimum medium for the shoot proliferation was MS＋6 -BA 1.00 mg·L^-1 ＋NAA 0.03 mg·L^-1 ＋ KT 2.50 mg·L^-1 ＋ GA3 0.05 mg·L^-1, and the proliferation multiples was 10. The optimal root induction medium was 1/2 MS ＋ NAA 0.25 mg·L^-1 ＋ sucrose 2%.

关 键 词：豆瓣绿 外植体 组织培养 

分 类 号：S688.9[农业科学—观赏园艺]