新疆野苹果组织培养体系的建立  

Studies on Tissue Culture System of Malus sieversii

作　　者：刘兵[1] 彭立新[1] 

机构地区：[1]天津农学院园艺系,天津300384

出　　处：《天津农学院学报》2011年第2期10-12,共3页Journal of Tianjin Agricultural University

基　　金：国家自然科学基金项目"盐胁迫下珠美海棠Na+/H+ Antiporter基因的分离与功能分析"(30671440)

摘　　要：以新疆野苹果带腋芽的幼嫩茎段为外植体进行组织培养研究,采用在木本植物组织培养上经常采用的消毒方法,并进行外植体消毒方法的比较研究,结果表明:新疆野苹果带腋芽的幼嫩茎段组织经70%乙醇15 s、1‰氯化汞10 m in、70%乙醇10 s消毒后效果最好;初代诱导培养最佳培养基为改良MS+0.5 mg/L 6-BA+0.1 mg/L NAA;继代增殖最佳培养基为改良MS+0.5 mg/L 6-BA;生根培养最佳培养基为1/2改良MS+1 mg/L NAA。The stem segments of Malus sieversii were used as explants to study the tissue culture. By sterilizing the explants with the common disinfection method on woody plants and comparing the effects with different methods, the results indicate that the best disinfection method was rinsing the explants with 70% alcohol for 15 s, then immersing them in 0.1% HgCl2 for 10 min, and finally immersing them in 70% alcohol for 10 s. The best induction medium was modified MS ＋0.5 mg/L 6 - BA ＋0.1 mg/L NAA, the optical buds multiplication medium was modified MS ＋ 0.5 mg/L 6 - BA, and the best rooting medium was modified MS ＋ 1 mg/L NAA.

关 键 词：新疆野苹果 茎段 组织培养 

分 类 号：S661.1[农业科学—果树学]