TLR4和TLR9mRNA在麻风患者外周血单核细胞中的表达  

The Expression of TLR4 and TLR9 mRNA on Peripheral Blood Mononuclear Cells (PBMC) in Patients with Leprosy

作　　者：罗丽[1] 刘源[1] 张信江[1] 龚少智[1] 容荣[1] 贾长莎[1] 孔晓东[1] 鲁东平[1,2] 杨宪鲁[1] 张庆波[1] 王宝仁[3] 刘放鸣 

机构地区：[1]遵义医学院附属医院皮肤科,贵州遵义563000 [2]深圳市宝安区慢性病防治院皮肤科,广州深圳518113 [3]贵州毕节地区医院皮肤科,贵州毕节551500 [4]贵州毕节地区疾病预防控制中心,贵州毕节551500

出　　处：《中国皮肤性病学杂志》2011年第7期503-505,共3页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金(30960350);贵州省省长资金[2006,(48)号]

摘　　要：目的观察TLR4和TLR9mRNA在麻风患者外周血单核细胞中的表达情况。方法运用SYBRGreen荧光定量RT-PCR技术检测麻风现症患者(30例)与已痊愈患者(30例)外周血单核细胞中TLR4和TLR9mRNA的表达水平,并与正常人作比较。结果 TLR9mRNA在麻风患者外周血单核细胞中的表达明显高于已痊愈组和正常对照组(P均<0.01),但痊愈组和正常对照组的差异不显著;且TLR4mRNA在麻风现症患者外周血单核细胞中的表达与痊愈组和正常对照组的差异也无统计学意义(P均>0.05)。结论 TLR9mRNA在麻风患者外周血单核细胞的表达明显升高,提示TLR9mRNA可能是麻风杆菌的特异识别受体,TLR9可能参与了机体感染麻风分枝杆菌后的免疫反应。TLR4是否参与了机体感染麻风分枝杆菌后的免疫反应尚有待于进一步论证。Objective To study the expression of TLR4 and TLR9 mRNA on peripheral blood mononuclear cells （PB- MC ） in patients with leprosy. Methods A total of 90 subjects including 30 with leprosy, 30 recovered from leprosy and 30 normal controls were enrolled in this study. SYBR Green fluorescence quantitative RT-PCR was used to assess the expression of TLR4 and TLR9 mRNA in PBMC of them. Results Expression of TLR9 mRNA in leprosy patients was significantly higher than that in both the recovered and normal controls （P 〈 0.01 ）, There was no significant difference between the recovered and normal control （P 〉0.05 ） ; The expression of TLR4 mRNA had no significant difference among leprosy, recovered and normal control （ both P 〉 O. 05）. Conclusion The expression of TLR9 mRNA was significantly higher on the PBMC in leprosy patients, suggesting that TLR9 may be the specific recognizing receptor participated the immune response in Mycobacterium leprae. Further studies are required to reveal the mechanism＇by which Mycobacterium leprae activates TLR4 mRNA.

关 键 词：TOLL样受体4 TOLL样受体9 外周血 麻风 

分 类 号：R755[医药卫生—皮肤病学与性病学]