姜黄素对HaCaT增殖的抑制作用及对Notch-1,Bax和Bak表达的影响  

Effects of Curcumin on Proliferation Inhibition and the Expression of Notch-1,Bax and Bak in HaCaT Cells

作　　者：曹毅[1] 王莉[1] 杨晓红[1] 陶茂灿[1] 罗宏宾[1] 

机构地区：[1]浙江中医药大学第一附属医院皮肤科,浙江杭州310006

出　　处：《中国皮肤性病学杂志》2011年第7期506-508,共3页The Chinese Journal of Dermatovenereology

基　　金：浙江省自然基金(Y2100759)

摘　　要：目的研究姜黄素对HaCaT细胞抑制增殖的作用及其作用机制。方法倒置显微镜下观察不同浓度姜黄素作用后HaCaT细胞的形态学改变;MTT法检测不同浓度姜黄素对HaCaT细胞抑制增殖的作用;Western blot法检测Notch-1,Bax和Bak蛋白的表达情况。结果 0.01μmol/L姜黄素处理HaCaT细胞后的细胞活性与正常组的差异无统计学意义(P>0.05),>0.1μmol/L的姜黄素处理HaCaT细胞后,其增殖受到抑制,且均与姜黄素有浓度依赖关系(P均<0.05)。Western bolt法检测结果显示,Notch-1的表达逐渐降低,Bak和Bax的表达逐渐升高。结论姜黄素对HaCaT细胞的增殖具有抑制作用,并可诱导细胞凋亡。推测姜黄素可能通过下调Notch-1受体的表达而改变细胞凋亡蛋白的表达,从而发挥抑制角质形成细胞增殖的作用.Objective To investigate the mechanism of the anti-proliferation effects of curcumin on HaCaT cells. Methods The inverted microscope was used on morphology of HaCaT cells under different concentrations of curcumin. Growth inhibition rates of HaCaT cells were measured with MTT method. The expression of Notch-1, Bax and Bak protein were detected by western blot assay. Results Reasults of MTF showed that growth inhibition rates of HaCaT cells of 0.01μmol/L curcumin group has no difference compared to the control group （P 〉 O. 05）. After HaCaT cells were treated with curcumin （ 〉 0. 1μmol/L）, cell growth was significantly inhibited in a dose-dependent manner （P 〈 0.05 ）. The percentage of the apoptotic cells were significantly increased after the treatment（ P 〈 0.05 ）. Detection western blot showed that both the expression of Bax and inducing Bak were increased and Notch-1 was decreased. Conclusion Curcumin no/only in- hibits proferaation but also induces apoptosis of HaCaT cells. Initially, curcumin may play the role of inhibiting proliferation on HaCaT cells by reduceing the expression of Notch-1 receptor and apoptosis protein.

关 键 词：姜黄素 HACAT NOTCH-1 BAX BAK 凋亡 

分 类 号：R986[医药卫生—药品] R758.63[医药卫生—药学]