力竭游泳运动对肠组织MDA、Free-SH、ATP含量、Na~＋-K~＋-ATPase和Ca^(2＋)-ATPase活性的影响  

Effects of Exhaustive Swimming on Lipid Peroxidation,Free Thiol Group,ATP Content,Na^+-K^+-ATPase and Ca^(2+)-ATPase Activity in the Rat's Intestine Tissue

作　　者：李风华[1] 王宝明[2] 衣雪洁[2] 

机构地区：[1]北京工业职业技术学院,北京100042 [2]沈阳体育学院运动人体科学学院,辽宁沈阳110102

出　　处：《沈阳体育学院学报》2011年第3期72-76,共5页Journal of Shenyang Sport University

摘　　要：探讨力竭性运动引起的氧化应激对肠功能的影响及运动性肠功能紊乱的原因,32只SD雄性大鼠随机分为4组:即对照组(C);运动后即刻组EX;运动后30min组(EX30);运动后60min组(EX60),测定力竭性游泳后不同时相肠组织匀浆MDA、游离巯基和ATP含量。结果:运动后肠组织MDA含量在运动后30min、60min显著性增加(P<0.01);运动后Free-SH含量在运动后30min(P<0.05)和运动后60min后(P<0.01)显著下降;运动后30min组ATP含量显著下降(P<0.01);运动后30min,Ca2+-ATPase活性下降(P<0.05);运动后60min,Na+-K+-AT-Pase和Ca2+-ATPase活性均显著下降(P<0.05)。结论:运动源性自由基产生增加,使肠组织中游离巯基被氧化,导致ATP含量下降,Na+-K+-ATPase和Ca2+-ATPase活性下降,可能是造成运动性肠功能紊乱的重要因素之一。In order to investigate the effects of oxidative stress on intestines function and the exercise-induced intestines function disorder,32 male SD rats were divided into four groups（control group,immediate postexercise group,30 min postexercise group and 60 min postexercise group）.We measured contents of MDA,FREE-SH and ATP contents in rats intestine tissues.The result showed that MDA contents had a significant increase at 30 min and 60 min after exhaustive swimming while FREE-SH contents had a remarkable decrease in the 30 min postexercise group and 60 min postexercise group.At the same time,ATP content decline significantly 30 min postexercise group.Ca2＋-ATPase activity declines remarkably 30min after exhaustive swimming exercise（P0.05）.Na＋-K＋-ATPase and Ca2＋-ATPase activity decrease significantly in 60 min postexercise group（P0.05）.The result suggested that increase of exercise-induced free radicals led to FREE-SH of intestine cross-link,which resulted in the decline of ATP content,Na＋-K＋-ATPase and Ca2＋-ATPase activity.These might be important factors of exercise-induced intestine disorder.

关 键 词：力竭性游泳 丙二醛 游离巯基 ATP 肠组织 Na+-K+-泵 Ca2+-泵 

分 类 号：G804.54[文化科学—运动人体科学]