地鳖纤溶活性蛋白对荷瘤小鼠肿瘤微血管密度及血管内皮细胞生长因子表达的影响  

Effect of Eupolyphaga Fibrinolytic Protein on Microvessel Density and the Expression of Vascular Endothelial Growth Factor in Mice Tumor

作　　者：曹付春[1] 韩雅莉[1] 陈冰[1] 刘浩[1] 丁鸿[1] 黄明星[1] 叶韵[1] 

机构地区：[1]广东工业大学轻工化工学院生物工程系,广东广州510006

出　　处：《中药材》2011年第5期676-679,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金项目(30772739);广东工业大学轻工化工学院"211工程"培育项目(20091015)

摘　　要：目的:研究地鳖纤溶活性蛋白(EFP)对H22和S180荷瘤小鼠肿瘤组织微血管密度(MVD)及血液中血管内皮细胞生长因子(VEGF)的影响,以判定EFP是否具有抗肿瘤新生血管生成作用。方法:建立H22荷瘤小鼠动物模型,采用免疫组化方法标记CD34,检测了EFP对小鼠H22和S180肿瘤组织MVD的作用情况,采用双抗体夹心ELISA法检测H22和S180荷瘤小鼠血清中VEGF含量。结果:与对照组相比,EFP可显著降低MVD及VEGF的表达。结论:EFP有抑制肿瘤新生血管形成的作用。Objective：To study the effect of Eupolyphaga fibrinolyric protein（EFP） on microvessel density（MVD） and the expression of vascular endthelial growth factor in transplantation S180 and H22 mice.Methods：The MVD in tumor was measured with immunohistochemical SP method and the VEGF level in serum was measured with ELISA method.Results： Compared with the control group,EFP could significantly reduce the microvessel density and decrease the expression of vascular endothelial growth factor.Conclusion：EFP has the effect of anti-angiogenesis.

关 键 词：地鳖纤溶活性蛋白 H22细胞 S180细胞 微血管密度 血管内皮细胞生长因子 

分 类 号：R285.5[医药卫生—中药学]