蛇床子素对腰椎间盘突出致坐骨神经痛大鼠的作用及其机制研究  

Effects and Mechanisms of Osthole on Sciatica Induced by Lumber Disc Herniation

作　　者：魏明[1] 张劲军[1] 贺秋兰[1] 王磊[2] 任政华[2] 孙来保[1] 刘先国[3] 

机构地区：[1]中山大学附属第一医院麻醉疼痛专科 [2]中山大学生命科学院 [3]中山大学疼痛研究中心,广东广州510080

出　　处：《中药材》2011年第5期746-750,共5页Journal of Chinese Medicinal Materials

基　　金：中山大学医院与生命科学院合作基金(162011);广东省自然科学基金(8161008901000194)

摘　　要：目的:研究蛇床子素对腰椎间盘突出致坐骨神经痛大鼠的作用及其机制。方法:54只SD雄性大鼠随机分为6组,模型组(n=12):L5背根神经节(dorsal root ganglion,DRG)移植自体髓核并行硬膜外腔置管。溶剂对照组(n=12):在模型组的基础上,于术后第6天予50μL吐温-80硬膜外腔注射。术后第2天给药组(n=6)、术后第6天给药组(n=12)、术后第13天给药组(n=6)、术后第20天给药组(n=6):分别在模型组的基础上,术后第2、6、13、20天予50μL2%蛇床子素硬膜外腔注射。各组取6只大鼠分别于术前1天及术后第1、3、5、7、14、21、28天,以及给药或溶剂前即刻和随后1 h行一般行为学观察和50%机械性撤足阈值(50%paw withdrawal threshold,50%PWT)测定,另外模型组、溶剂对照组及术后第6天给药组剩余6只大鼠于术后第7天检测术侧L5 DRG中NOS和COX-2的表达。结果:所有大鼠均未出现跛行、自噬等现象。造模后大鼠50%PWT显著下降(P<0.05)。给药后术后第2天给药组和术后第6天给药组50%PWT比模型组、溶剂对照组显著升高(P<0.05),恢复到造模前水平(P>0.05);术后第13天给药组和术后第20天给药组仅给药后1 h50%PWT比模型组、溶剂对照组显著升高(P<0.05),恢复到造模前水平(P>0.05);但随后时间大鼠50%PWT又回降到造模后水平(P>0.05),与模型组、溶剂对照组无异(P>0.05)。NOS与COX-2阳性细胞数术后第6天给药组给药后均比模型组和对照组显著降低(P<0.05)。结论:50μL2%蛇床子硬膜外腔注射在早期(术后第2天和术后第6天给药)可完全抑制椎间盘突出致坐骨神经痛大鼠模型的机械痛敏,在晚期(术后第13天和术后第20天)仅表现出一过性的抑制作用;其第6天对椎间盘突出致坐骨神经痛大鼠模型产生作用的机制可能与抑制DRG中COX-2和NOS的表达有关。Objective：To Study the effects of osthole on sciatica induced by lumber disc herniation and its mechanisms.Methods：54 male SD rats were randomly divided into 6 groups.Model（M） group（n=12）：Autologous nucleus pulposus was harvested from the tail and applied to the L5 dorsal root ganglion（DRG） and epidural space.Epidural catheterization was performed.Control（C） group（n=12）：On the basis of nucleus pulposus group,50 μL tween-80 was administered epidurally on the day 6th after surgery.T2（n=6）,T6（n=12）,T13（n=6） and T20（n=6） group：50 μL 2% osthole was administered epidurally on the 2th,6th,13th day and 20th after surgery respectively.General behaviors were observed and 50% paw withdrawal threshold（50% PWT） was measured 1 day before surgery,on the 1st,3th,7th,14th,21th,28th day after surgery,immediately before and 1 hour after osthole or tween-80 administration in each group.On the 7th day after surgery,the left L5 DRGs were obtained for detecting the expression of NOS and COX-2 in M,C and T6 group with 6 rats.Results： No lameness or autophagy was oberserved.50%PWT decreased after surgery（P0.05）.In T2 and T6 group,50% PWT after osthole administration were significantly higher than those of M group and C group（P0.05）,which recovered to the same level as 1 day before surgery（P0.05）.In T13 and T20 group,50% PWT 1 hour after osthole administration were significantly higher than those of M group and C group（P0.05）,which recovered to the same level as 1 day before surgery（P0.05）,but on days after 1 hour after administration,there was no significant difference when 50%PWT compared with M group or nucleus C group（P0.05）.NOS positive cells and COX-2 positive cells were no significant difference when M group compared with C group（P0.05）.But these positive cells in T6 group were significantly lower than those of M group and C group（P0.05）.Conclusion： 50 μL 2% osthole could completely inhibit the mechanical allodynia in the rat model of sciatica

关 键 词：蛇床子素 髓核 坐骨神经痛 一氧化氮合酶 环氧合酶-2 

分 类 号：R285.5[医药卫生—中药学]