日本血吸虫虫卵破坏小鼠脾脏结构的作用  

Destructive effect of eggs of Schistosoma japonicum on spleens in C57BL/6 mice

作　　者：王燕娟[1] 徐馀信[1] 胡媛[1] 沈玉娟[1] 李佩[2] 周何军[1] 曹建平[1] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所,卫生部寄生虫病原与媒介生物学重点实验室,世界卫生组织疟疾、血吸虫病和丝虫病合作中心,上海200025 [2]上海交通大学

出　　处：《中国血吸虫病防治杂志》2011年第3期285-287,I0002,共4页Chinese Journal of Schistosomiasis Control

基　　金：国家自然科学基金(30872212、30801047、30771880);国家重大科技专项(2009ZX10004-201、2008ZX10004-002);国家高技术研究发展计划(863计划)(2006AA02Z444)

摘　　要：目的观察日本血吸虫虫卵对脾脏结构的病理影响。方法 C57BL/6小鼠分别经尾静脉注射日本血吸虫虫卵或虫卵可溶性抗原,经剖腹手术后向小鼠脾脏注射虫卵,4周后剖杀小鼠分离脾脏,石蜡切片苏木素伊红染色观察脾脏结构。以单性和双性尾蚴感染小鼠,9周后剖杀比较组间脾脏质量,石蜡切片观察各组小鼠脾脏结构。结果尾静脉注射虫卵小鼠脾脏淋巴滤泡数目减少,边缘区模糊难辨。手术将虫卵注射入小鼠脾脏4周后,虫卵注射部位淋巴滤泡萎缩甚至消失。单性尾蚴感染小鼠脾脏质量(0.15±0.01)g,显著低于双性尾蚴感染组(0.41±0.03)g(P<0.01)。苏木素伊红染色脾脏切片显示单性尾蚴感染组小鼠脾脏淋巴滤泡未被破坏,而双性尾蚴感染组脾脏淋巴滤泡消失。结论日本血吸虫虫卵对C57BL/6小鼠脾脏结构有破坏作用。Objective To observe the destructive effect of eggs of Schistosoma japonicum on the splenic structure in C57BL/6 mice. Methods The C57BL/6 mice were injected intravenously with eggs of S. japonicum or soluble egg antigen （SEA）, or the eggs were injected surgically into the spleens of the mice. Four weeks later, the mice were sacrificed, the splenic paraffin sections were stained with H E and the splenic structures were observed. Other two groups of mice were infected with single-sex and both sexes of schistosome cercariae, respectively. Nine weeks after the infection, the mice were sacrificed, and the splenic structures were observed and compared among the groups aforementioned. Results The splenic structure of mice injected intravenously with eggs were destroyed, and characterized with decreased number of lymphoid follicles and blurred marginal zone. Lymphoid follicles around the eggs injected into the spleen were also seriously depauperated. The splenic weight （0.15±0.01） g of the mice infected with single-sex cercariae significantly less than that （0.41±0.03） g of the mice infected with both sexes cercariae （P0.01）. However, the splenic structure of the mice infected with single-sex cercariae kept integrated. Conclusion Eggs of S. japonicum have a destructive effect on the splenic structure in C57BL/6 mice.

关 键 词：日本血吸虫 脾脏 虫卵 可溶性虫卵抗原 小鼠 

分 类 号：R383.24[医药卫生—医学寄生虫学]