Determination of Ciprofloxacin in Human Plasma and Urine by Precolumn Derivatization High Performance Liquid Chromatography with Fluorescence Detection  

作　　者：Ulu, Sevgi Tatar 

机构地区：[1]Department of Analytical Chemistry, Faculty of Pharmacy, University of lstanbul, 34416 Istanbul, Turkey

出　　处：《Chinese Journal of Chemistry》2011年第6期1256-1260,共5页中国化学（英文版）

摘　　要：A sensitive, simple and selective high-performance liquid chromatographic （HPLC） method was developed for the determination of ciprofloxacin in biological fluids. The method is based on the reaction between the drug and 4-chloro-7-nitrobenzofurazan （NBD-C1） in borate buffer of pH 9.0 to yield a highly fluorescent derivative that is measured at 535 nm after excitation at 464 nm. The calibration curves were linear over the concentration ranges of 25--3000 and 50--3000 ng·mL -1 for plasma and urine, respectively. The mean recovery of ciprofloxacin from plasma and urine was 98.37% and 98.40%, respectively. The method was found to be sensitive, precise, accurate, and reproducible. All of the validation parameters were within the acceptance range.A sensitive, simple and selective high-performance liquid chromatographic （HPLC） method was developed for the determination of ciprofloxacin in biological fluids. The method is based on the reaction between the drug and 4-chloro-7-nitrobenzofurazan （NBD-C1） in borate buffer of pH 9.0 to yield a highly fluorescent derivative that is measured at 535 nm after excitation at 464 nm. The calibration curves were linear over the concentration ranges of 25--3000 and 50--3000 ng·mL -1 for plasma and urine, respectively. The mean recovery of ciprofloxacin from plasma and urine was 98.37% and 98.40%, respectively. The method was found to be sensitive, precise, accurate, and reproducible. All of the validation parameters were within the acceptance range.

关 键 词：high-performance liquid chromatographic （HPLC） CIPROFLOXACIN DERIVATIZATION 4-chloro-7-nitrobenzofurazan validation biological fluids 

分 类 号：TQ464.7[化学工程—制药化工] O657.72[理学—分析化学]