载脂蛋白E基因敲除及高脂饮食小鼠皮质额叶区形态学及Mortalin表达的变化  

Change of apolipoprotein E gene knock-out and high-fat diet on morphology and mortalin expression in mice frontal lobe neurons

作　　者：杨平[1,2] 周祎[3] 刘娟[1] 黄大可[1] 桂丽[1] 汪渊[1] 贾雪梅[1] 

机构地区：[1]安徽医科大学基础医学院,合肥230032 [2]安徽医科大学医学心理学系,合肥230032 [3]安徽医学高等专科学校,合肥230601

出　　处：《安徽医科大学学报》2011年第7期615-619,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:30570750);安徽省教育厅自然科学基金(编号:2006KJ324B)

摘　　要：目的观察载脂蛋白E基因敲除(ApoE KO)及高脂饮食(HF)后小鼠额叶区形态学及Mortalin蛋白表达变化,以进一步探讨ApoE异常导致阿尔茨海默病及记忆损伤的可能机制。方法 10只野生型小鼠予普通饲料喂养作为对照(C)组,10只ApoE KO小鼠予普通饲料喂养作为KO组,10只ApoE KO小鼠予高脂饲料喂养作为KO-HF组。小鼠造膜成功后称重,取血检测血脂,取小鼠脑组织石蜡包埋切片,分别进行HE染色、尼氏染色、免疫组化染色及计算机图像分析。结果 KO组体质量、总胆固醇、甘油三酯及低密度脂蛋白胆固醇含量与C组相比明显升高,KO-HF组升高更明显(P<0.05);KO组及KO-HF组小鼠大脑皮质额叶神经元内尼氏体平均光密度值较C组减少,Mortalin平均光密度值较C组升高(P<0.01)。结论 ApoE KO及HF可致额叶区神经元内尼氏体减少,Mortalin蛋白表达上调,该蛋白可能与ApoE异常导致阿尔茨海默症认知功能障碍有着密切的关系。Objective To investigate the effect of apolipoprotein E gene knock-out（ApoE KO）and high-fat diet（HF） on morphology and the expression of mortalin in neurons of mice cortex frontal lobe,and explore the impact ofthese factors on memory and Alzheimer′s disease.Methods Ten wild-type and 10 ApoE KO mice were fed with common chow as the control（C） group and the KO group respectively,while 10 ApoE KO mice were fed with HF as the KO-HF group.12 weeks later,the weight and the lid of these mice were measured.The brain tissues were observed by HE staining,nissl staining,immunohistochemistry staining and image analysis by computer.Results In the ApoE KO group,weight,total cholesterol,triglyceride,low-density lipoprotein cholesterol were higher than those in the C group,and these changes were more significant in KO-HF group.The average optical density of the nissl body was higher in the KO group and in KO-HF group than those in the C group（P0.01）.The average optical density of the mortalin in the KO group and in KO-HF group were higher than those in the C group（P0.01）.Conclusion ApoE KO and high-fat diet can reduce nissl body,and improve the expression of mortalin.The mortalin may be involved in the pathogenesis of Alzheimer′s disease.

关 键 词：载脂蛋白E类 小鼠 基因敲除 额叶/病理学 

分 类 号：R341[医药卫生—基础医学] R329.481