腹腔感染大鼠机体免疫状态变化  

Immune Imbalance of Rats with Severe Abdominal Infection

作　　者：喻文立[1] 崔乃强[2] 傅强[2] 李东华[3] 

机构地区：[1]天津市第一中心医院麻醉科,天津300192 [2]天津市南开医院外科 [3]天津市中西医结合急腹症研究所

出　　处：《中国中西医结合外科杂志》2011年第3期276-279,共4页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：国家重点基础研究发展计划(973计划)(2009CB522703)

摘　　要：目的:观察腹腔感染大鼠的机体免疫状态变化,研究清热解毒方剂对机体细胞免疫失衡的影响。方法:采用人工胃液联合大肠杆菌腹腔内注射的方法建立大鼠腹腔感染模型,取Wistar大鼠120只随机分为对照组、模型组、清热解毒方剂组,造模后12h、24h、48h、72h经腹主动脉取血,采用流式细胞仪检测全血中辅助性T细胞1/2(Th1/Th2)比值以及调节性T细胞(Treg)比例的变化。结果:与正常对照组比较,模型组Th1/Th2比值在造模后12h明显升高,造模后48、72h明显降低(P<0.05或P<0.01),而Treg比例在造模后12h明显下降,随后明显升高(P<0.05或P<0.01),清热解毒方剂组Th1与Th2比值以及Treg比例升高和降低的幅度较模型组明显减低。在模型组和清热解毒方剂组,Th1/Th2比值与Treg比例呈线性负相关(P<0.05)。结论:腹腔感染大鼠细胞免疫功能抑制,表现为Th1/Th2比值减少,其机制与肠源性内毒素介导Treg比例和活性增加有关;清热解毒方剂可能通过调节Treg比例来减少Th1向Th2的漂移,从而起到维持机体免疫平衡的作用。Objective To investigate the cellular immune function changes during sever abdominal infection,the mechanism of cellular immune imbalance and the effect of Qingrejiedu （清热解毒） decoction on the immune balance.Methods The rat model of severe abdominal infection was established by intra-abdominal E.coli administration.One hundred and 20 rats were divided into 3 groups randomly,including the control group,the model group and the treatment group.At the time of 12 h,24,48 h after the operation,blood sample in the abdominal aorta were collected.Th1/Th2 ratio and Regulatory T cells （Treg） percentage of CD4＋ T cell was determined by means of flow cytometry respectively.Results The level of Th1/Th2 ratio increased in the early period of sepsis （12 h） but decreased in the late period of sepsis （from 24 to 72 h） in the model group;while the percentages of Treg in CD4＋ T cells were significantly decreased at 12 h and 24 h and increased at 48 h and 72 h in the model group compared with those in the control group.The trends of these parameters were similar in the treatment group,but the magnitudes were smaller.There were negative correlations between the ration of Th1/Th2 and the percentage of Treg both in the model group and in the treatment group.Conclusion There is a dissonance in immunological function in rats with abdominal infection,including a depression in T cell immunological function,whereas Treg may participate in sepsis-induced immunoparalysis.Qingrejiedu（清热解毒） herbal decoction can obviously ameliorate the immunological disturbance in rats with severe abdominal infection.

关 键 词：腹腔感染 清热解毒方剂 T辅助细胞 调节性T细胞 

分 类 号：Q95-33[生物学—动物学] R658.3[医药卫生—外科学]