亚急性1，2-二氯乙烷染毒对小鼠行为及脑神经递质含量的影响  

Influence of 1, 2-dichloroethane on open field behavior and levels of neurotransmitters in brain of mice

作　　者：齐莹[1] 石磊[1] 高岚岳[1] 王高阳[1] 李革新[1] 吕秀强[1] 金亚平[1] 

机构地区：[1]中国医科大学公共卫生学院劳动卫生教研室,沈阳110001

出　　处：《中华劳动卫生职业病杂志》2011年第6期413-416,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的探讨亚急性1，2-二氯乙烷（1,2-dichloroethane，1，2-DCE）染毒对小鼠行为及脑神经递质含量的影响，为揭示1,2-DCE神经毒性机制提供参考。方法将昆明种小鼠随机分为4组：对照组和低、中、高剂量1,2-DCE染毒组（225、450、900mg/m^3），每组8只小鼠，静式吸入染毒10d，每天染毒3．5h。最后一次染毒结束后立即进行旷场试验，实验结柬后处死小鼠，快速取大脑组织，采用高效液相色谱法（HPLC）检测脑组织中谷氨酸（Glu）、天冬氨酸（Asp）及γ-氨基丁酸（GABA）的含量。结果各染毒组小鼠脑组织中Asp和Glu含量随染毒剂量的增加而升高，且各染毒组小鼠的Glu含量[低、中、高剂量染毒组分别为（67．69±9．89）、（67．99±6．23）、（71．16±5．96）μmol／gPro]与对照组[（50．78±5．15）μmol/gPro]比较，差异均有统计学意义（P〈0．01）；低剂量染毒组小鼠脑组织中GABA含量[（8．08±2．37）μmol／gPro]较对照组[（12．83±3．36）μmol／gPro]明显降低，但高剂量组[（19．87±5．30）μmol/Pro]与对照组比较，明显升高，差异均有统计学意义（P〈0．05，P〈0．01）。低剂量1,2-DCE染毒对小鼠的行为有兴奋作用；而高剂量1，2-DCE染毒对小鼠的探索性及运动性行为有抑制作用。结论亚急性1,2-DCE染毒可引起小鼠脑组织中氨基酸类神经递质含量及比值的变化，进而导致出现行为的改变，这可能是1,2-DCE神经毒性作用的机制之一。Objective To explore the effects of 1,2-dichloroethane （1,2-DCE） on the behavior and the brain neurotransmitter levels in mice. Methods Thirty mice were randomly divided into four groups, which were control group and groups of low, meddle and high exposure （225, 450 and 900 mg/m^3） to 1,2-DCE for 10 days （3.5 h a day） by inhalation. After the last exposure, the open field test was performed immediately.After exposure all mice were killed and the brain tissues were taken up rapidly. The levels of aspartate （Asp）, glutamate （Glu） and gamma-aminobutyric acid （GABA） in the brain were detected by high performance liquid chromatography （ HPLC ）. Results Levels of Asp and Glu in all exposure groups increased with doses. As compared to the control group, levels of Glu in all exposure groups increased significantly （P〈0.05）. Levels of GABA in the low exposure group were significantly lower than those in control group, but those in the high exposure group were significantly higher than those in control group. The results of the open field test showed that effect of low exposure to 1,2-DCE on the behavior was stimulant, but the high exposure to 1,2-DCE inhibited behavior of exploration, excitement and sport. Conclusions Subacute exposure to 1,2-DCE could result in the change of amino acid neurotransmitter content and ratio in the brain, thereby change the behavior of mice appeared, which might be the mechanism of neurotoxicity caused by 1,2-DCE in part.

关 键 词：1 2-二氯乙烷 小鼠 旷场实验 神经递质 

分 类 号：R285.5[医药卫生—中药学]