人参皂甙Rb1对马桑内酯致痫大鼠海马组织蛋白质组的影响  

Effects of ginsenoside Rb1 on proteome from hippocampus of rats with epilepsy induced by Coriaria lactone

作　　者：徐倩[1] 张春来[1] 杨烨[2] 张春燕[2] 赵春玲[1] 

机构地区：[1]泸州医学院生理教研室,四川泸州646000 [2]泸州医学院生物化学教研室,四川泸州646000

出　　处：《中国病理生理杂志》2011年第6期1218-1221,共4页Chinese Journal of Pathophysiology

基　　金：四川省教育厅资助项目(No.2006C010);泸州医学院重点资助项目

摘　　要：目的:运用双向电泳技术获得可能与马桑内酯(CL)癫痫发作及人参皂甙Rb1(GRb1)神经保护作用有关的蛋白质成分,探讨海马组织蛋白质在癫痫发生发展过程中的作用和地位以及GRb1对其的影响。方法:分别提取对照组、CL致痫组和GRb1+CL致痫组动物海马组织蛋白,双向电泳分离,ImageMaster 2D Platinum 5.0软件进行差异表达蛋白质组分析,基质辅助激光解吸附离子化飞行时间质谱(MALDI-TOF-MS)鉴定蛋白质。结果:成功鉴定出6个蛋白质分别是:脑肌酸激酶(brain creatine kinase)、内吞蛋白A1(endophilin-A1)、UPF0628蛋白C10orf96同源物(UPF0628 protein C10orf96 homolog)、细胞色素P-450(cytochrome P-450)、光导素样蛋白(phosducin-like protein)及桥整合蛋白3(bridging integrator 3)。其中前3个蛋白质在GRb1+CL致痫组表达低于CL致痫组,后3个蛋白质在GRb1+CL致痫组表达低于对照组。结论:CL致痫组、GRb1+CL致痫组和对照组表达的蛋白质存在明显差异。鉴定出的6个差异表达的蛋白质可能与GRb1的神经保护作用有关,其中brain crea-tine kinase、endophilin-A1和UPF0628 protein C10orf96 homolog可能与CL所致癫痫发作有关。AIM：To explore the mechanism of development of Coriaria lactone（CL）-induced epilepsy and the neuroprotective effects of ginsenoside Rb1（GRb1） on the process of epilepsy by identifying the proteins related to CL and GRb1 in rat hippocampus with two-dimensional electrophoresis（2-DE） technology.METHODS： The rat model of epilepsy was induced by CL.The rats in control group,CL epileptic group and GRb1 ＋CL epileptic group were decapitated and the hippocampus were collected.Two-dimensional electrophoresis was applied to separate the proteins from each group.Analysis of 2-DE maps was used to determine differential expression of proteins by ImageMaster 2D Platinum 5.0,and some differentially expressed protein spots were picked up for further identification by matrix-assisted laser sorption ionization time-of-flight mass spectrometry（MALDI-TOF-MS）.RESULTS： Six proteins were successfully identified.Among these,the expression of brain creatine kinase,endophilin-A1 and UPF0628 protein C10orf96 homolog was lower in GRb1＋CL epileptic group than those in CL epileptic group.The expression of cytochrome P-450,phosducin-like protein and bridging integrator 3 protein was lower in GRb1＋CL epileptic group than those in control group.CONCLUSION： The protein expression profiles are significantly different among control group, CL epileptic group and GRb1＋CL epileptic group.The identified proteins may be related to the neuroprotective effects of GRb1.Among these,brain creatine kinase,endophilin-A1 and UPF0628 protein C10orf96 homolog may be related to CL-induced seizures.

关 键 词：马桑内酯 癫痫 人参皂甙RB1 蛋白质组 大鼠 

分 类 号：R338[医药卫生—人体生理学] Q593[医药卫生—基础医学]