黄脊竹蝗5个地理种群线粒体16S rRNA基因片段的序列分析  

Fragment sequences analysis of mtDNA-16S rRNA gene among 5 geographic populations of Rammeacris kiangsu

作　　者：姜石生[1] 贺一原[1] 朱道弘[2] 曹元清 

机构地区：[1]中南林业科技大学,湖南长沙410004 [2]湖南第一师范学院,湖南长沙410004 [3]新宁县第四中学,湖南新宁422709

出　　处：《中南林业科技大学学报》2011年第7期115-119,共5页Journal of Central South University of Forestry & Technology

基　　金：国家自然科学基金项目(30371167)

摘　　要：摘要对湖南桃江、广东广宁、广西桂林、重庆永昌、四川长宁等5个黄脊竹蝗Rammeacris kiangsu Tsai地理种群线粒体16S rRNA基因进行测序,并经Clustal X同源排序后得506 bp序列。对获得的序列分析表明,A,T,G,C平均含量分别为32.0%,37.7%,17.6%,12.6%,其中保守位点数504个,变异位点数2个,自裔位点2个,所有碱基转换总数为1,颠换总数为1。利用MEGA4.0软件重建系统发生树,发现其中这5个黄脊竹蝗种群分化程度很低。从而可以说明其群体遗传多样性单一。Approximately 506 base pairs of mitochondrial 16S rRNA gene from 5 populations of Rammeacris kiangsu Tsai （Orthoptera：Aeridoidea） in Tao-jiang, Guang-ning, Gui-lin, Chang-ning, Yong-chang were sequenced. Of these sequences, average contents of A,T,G and C were 32. 0%,37. 8%,17. 6% and 12. 7%, respectively. In 506 bp sequences, there were 504 conserved sites, 2 variable sites and 2 singleton sites. A total of transition and transversion among mtDNA 16S rRNA gene were 1 and 1 respectively. The molecular phylogenetic trees were reconstructed by MEGA4. 0 software methods, it was found that there was a consistent evolutionary relationship among the 5 populations. The results show the genetic diversity in 5 populations of Rarnmeacris kiangsu was exceptionally low.

关 键 词：黄脊竹蝗 地理种群 16S rRNA遗传分化 遗传多样性 

分 类 号：X503.223[环境科学与工程—环境工程]