中华补血草和中亚滨藜叶片中盐激蛋白和dehydrin的研究  

STUDIES OF SALT STRESS PROTEINS AND DEHYDRIN IN ATRIPLEX CENTRALASTATICA AND LIMONIUM SINENSIS

作　　者：王洪燕[1] 刘箭[1] 石凤[2] 

机构地区：[1]山东师范大学生物学系,山东济南250014 [2]山东师范大学测试中心,山东济南250014

出　　处：《山东师范大学学报（自然科学版）》1999年第4期422-425,共4页Journal of Shandong Normal University(Natural Science)

基　　金：国家自然科学基金!(39770076)

摘　　要：应用二相电泳方法,分析中华补血草Limonium sinensis(Girard.) O.Kuthze.和中亚滨藜AtriplexcentralasiaticaIljin 这二种典型泌盐植物叶片中的蛋白组成变化,发现盐胁迫时,二种植物叶片中均有少量的盐激蛋白出现,其中中亚滨藜叶片中的蛋白质组成变化大一些.运用Western- blot 方法对这二种盐生植物叶片中的dehydrin蛋白进行检测,结果发现,无论是在正常环境下,还是高浓度盐渍环境下。The proteins in the leaves of the two kinds of halophytes,Atriplex centralasiatica Iljin and Limonium sinensis(Girard.)O.Kuthze,were analyzed by two-dimension electrophoresis when the palnts were suffered from salt stress.The results showed that there were a few of salt stress proteins in the two kinds of halophytes.By comparison,Atriplex centralasiatica Iljin expressed more salt stress proteins than Limonium sinensis did.Dehydrin,which could confer salt tolerance to crops,was not detected in these two kinds of halophytes with Western-Blot test,no matter whether the plants were treated by Hoagland's solution or Hoagland's solution added with 100,200,and 400mmol/L NaCl.

关 键 词：中华补血草 中亚滨藜 盐激蛋白 DEHYDRIN 

分 类 号：Q948.113[生物学—植物学]