等离子体处理乙丙交酯共聚物表面固定胶原的研究  

Investigation of Plasma Treatment and Collagen Immobilization on the Surface of Poly(lactid-glycolide acid)

作　　者：崔媛[1] 段潜[1] 李艳辉[1] 

机构地区：[1]长春理工大学,长春130022

出　　处：《材料工程》2011年第6期58-62,共5页Journal of Materials Engineering

基　　金：国家自然科学基金资助项目(50903009);吉林省科技发展计划项目(20100115)

摘　　要：选择等离子体对乙丙交酯共聚物(PLGA)表面进行活化处理来接枝胶原。通过测定等离子体处理后PLGA材料表面水接触角确定最佳处理功率为60W,时间5min。XPS分析确定胶原表面的羧基与PLGA表面等离子体激活的羟基发生了反应生成酯基,而使胶原接枝到PLGA表面。选用小鼠胚胎内皮成纤维细胞(3T3)在PLGA材料表面培养。实验定性观察确定胶原接枝有利于细胞的黏附和增殖。采用四甲基偶氮唑蓝比色法(MTT)对不同时期两种细胞生长情况进行定量分析,结果表明,接枝胶原的存在改善了细胞黏附作用,加快了细胞的增殖速度。For collagen is a major component of the extracellular matrix,when the surface of poly（lactid-glycolide acid）（PLGA） is grafted with collagen,cytocompatibility of PLGA will be improved.Plasma treatment is selected to immobilize collagen on the surface of PLGA.Optimum power and treating time of plasma are determined by contact angle test.The results indicate they are 60W and 5min,respectively.XPS analysis reveals that carboxy groups on collagen are reacted with actived hydroxyl groups on the surface of plasma treated PLGA to form ester groups.Then collagen is immobilized on PLGA.Cytocompatibility evaluation of collagen immobilized PLGA（COL-PLGA） was carried out by seeding 3T3 cells on the surface of COL-PLGA.Quanlitative and quantitative analysis proved that cell adhesion and proliferation are improved after collagen grafting.Quantitative result is obtained by MTT assay in cell living period.

关 键 词：PLGA 等离子体 胶原 

分 类 号：TQ225.24[化学工程—有机化工]