单核细胞趋化蛋白在肾盂尿路上皮癌中的表达及临床意义  

Expression of monocyte chemoattractant protein and MCP-1 gene in urothelial carcinoma of renal pelvis and its clinical significance

作　　者：白鑫[1] 高健刚[1] 侯四川[1] 孙小庆[1] 朱磊一[1] 

机构地区：[1]青岛市市立医院东院泌尿外科,山东青岛266071

出　　处：《临床肿瘤学杂志》2011年第6期530-533,共4页Chinese Clinical Oncology

基　　金：青岛市科技局基金资助项目(2007-K2D18)

摘　　要：目的探讨肾盂尿路上皮癌组织单核细胞趋化蛋白(MCP-1)及其基因表达情况,以及它们与临床病理特征的相关性。方法收集2007年1月至2008年1月肾盂尿路上皮癌患者20例,男性12例,女性8例,分别取其血液、癌及癌旁组织标本。同期选取30例非肿瘤患者,男性18例,女性12例,取血液标本。ELISA方法测定血浆MCP-1含量,免疫组化法检测组织MCP-1的表达。实时荧光定量PCR检测MCP-1 RNA水平。结果肾盂尿路上皮癌患者血浆MCP-1浓度为(173.4±82.1)pg/ml,高于非肿瘤患者的(91.8±34.6)pg/ml(P<0.05);高级别肾盂尿路上皮癌患者血浆MCP-1浓度为(254.1±125.8)pg/ml,高于低级别的(151.3±79.5)pg/ml。肾盂尿路上皮癌组织中MCP-1阳性表达率为90.0%(18/20),癌旁组织为65.0%(13/20),两组差异有统计学意义(P<0.01);高级别肾盂尿路上皮癌组织MCP-1阳性表达率为100.0%(4/4),高于低级别者的87.5%(14/16)。肾盂尿路上皮癌组织MCP-1总RNA和mRNA水平均高于其相应的癌旁组织(P<0.05)。结论肾盂尿路上皮癌组织较其癌旁组织的MCP-1基因表达上调,提示MCP-1可能在肾盂尿路上皮癌的发生及转移中发挥一定的作用。Objective To investigate the monocyte chemoattractant protein（MCP-1） gene expression of urothelial carcinoma of renal pelvis and adjacent normal tissues and the correlation of the incidence and pathological grading of urothelial carcinoma of renal pelvis.Methods Twenty cases of patients with urothelial carcinoma of renal pelvis（12 cases of male,8 cases of female） were taken the blood,carcinoma tissues and adjacent normal tissues.Thirty cases of non-cancer patients（18 cases of male,12 cases of female） as control group were taken blood samples.Expression of MCP-1 in plasma were detected by ELISA method quantitative determination,and the expression of MCP-1 in urothelial carcinoma of renal pelvis and adjacent normal tissues were investigated by immunohistochemical method.Real-time quantitative PCR was used to detect the expression of MCP-1 RNA.Results MCP-1 in plasma of urothelial carcinoma patients of renal pelvis was（173.4±82.1）pg/ml,higher than that of non-tumor group（91.8±34.6）pg/ml（P0.05）.Expression of MCP-1 in high-grade urothelial carcinoma of renal pelvis was（254.1±125.8）pg/ml,while in low-grade urothelial carcinoma of renal pelvis was（151.3±79.5）pg/ml.Immunohistochemistry showed that MCP-1 positive rate in urothelial carcinoma of renal pelvis was 90.0%（18/20）,and in adjacent normal tissues was 65.0%（13/20）,with significant differences（P0.01）.Positive expression rate of MCP-1 in high-grade urothelial carcinoma of renal pelvis was 100.0%（4/4）,while in low-grade urothelial carcinoma of renal pelvis was 87.5%（14/16）.Total RNA and mRNA levels of MCP-1 in the urothelial carcinoma of renal pelvis were statistically significant different compared with adjacent normal tissues group（P0.05）.Conclusion The upregulation of MCP-1 gene expression is likely to play an important role in the incidence and metastasis of the urothelial carcinoma of renal pelvis.

关 键 词：肾盂尿路上皮癌 单核细胞趋化蛋白 基因 

分 类 号：R737.11[医药卫生—肿瘤]