印迹壳低聚糖在小鼠体内的代谢与组织分布  

Metabolism and distribution of imprinted chitooligosaccharides in mice

作　　者：张晓菲[1,2] 刘丽宏[2] 丁春雷[2] 杨润涛[2] 赵长琦[1] 

机构地区：[1]北京师范大学生命科学学院,北京100875 [2]中国人民解放军第二炮兵总医院药剂科,北京100088

出　　处：《中国药理学与毒理学杂志》2011年第3期314-319,共6页Chinese Journal of Pharmacology and Toxicology

摘　　要：目的测定印迹水溶性壳低聚糖(CTS)在小鼠体内的代谢和分布。方法制备异硫氰酸荧光素标记的CTS(FITC-CTS),小鼠尾静脉iv给予FITC-CTS100 mg·kg-1,于10 min,0.5,1,4,8,12,24和48 h后行高效液相凝胶排阻色谱法(HPGPC)检测FITC-CTS在小鼠体内的含量,荧光分光光度法测定FITC-CTS在小鼠体内各组织中的分布。结果 FITC-CTS在血浆中快速清除,4 h其血浆浓度降低68%;在组织中60%分布到肾,30%分布到肝,几乎不分布到其他组织;24 h内体内90%通过尿液排出体外,主要为CTS原型。结论 CTS生物降解度低,主要分布到肾和肝,并原型快速通过尿液排出,体内基本无蓄积。OBJECTIVE To explore the metabolism and distribution of imprinted chitooligosaccharides（CTS） in mice.METHODS CTS were labeled by fluorescein isothiocyanate（FITC） before FITC-CTS 100 mg·kg^-1 were iv given to mice.At 10 min,0.5,1,4,8,12,24 and 48 h after administration,serum and urine samples were collected and FITC-CTS were detected by fluorospectrophotometer and high performance gel permeation chromatography（HPGPC）.RESULTS FITC-CTS was metabolized fast from plasma,and FITC-CTS concentration declined by 68% at 4 h.Sixty percentage FITC-CTS were distributed in kidneys,30% in the liver,and hardly distributed in other organs.Ninty percentage FITC-CTS were excreted from urine in its original form after 24 h.CONCLUSTION CTS are low biodegradable,mainly distribute in kidneys and liver,and totally excrete from urine in original form.

关 键 词：壳低聚糖 代谢 组织分布 

分 类 号：R965[医药卫生—药理学]