主动载药法制备槐定碱脂质体  

Preparation of Sophoridine Liposomes by Active Loading Method

作　　者：胡鹏翼[1] 郑琴[1] 杨明[1] 伍振峰[1] 岳鹏飞[1] 王芳[1] 

机构地区：[1]江西中医学院现代中药制剂教育部重点实验室,南昌330004

出　　处：《中国实验方剂学杂志》2011年第13期5-8,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：十一五重大新药创制项目(2009ZX09310-005);江西省自然科学基金项目(2008GQY0017);江西中医学院创新人才课题(JZ0909)

摘　　要：目的:考察槐定碱脂质体不同制备方法对其包封率的影响。方法:采用注入、薄膜分散、逆向蒸发等被动载药法和硫酸铵梯度、pH梯度结合逆向等主动载药法制备槐定碱脂质体,用高速冷冻离心-HPLC法测定脂质体包封率。结果:被动载药法制备的脂质体包封率较低,主动载药法中pH梯度结合逆向法制备脂质体包封率为93.27%。结论:采用主动载药法可制得包封率较高的槐定碱脂质体。Objective：To study the influence of different preparation methods on the entrapment efficiency.Method： Sophoridine liposomes was prepared by passive loading method and active loading method.The entrapment efficiencies were determined by high speed freeze centrifugation-HPLC method.Result： The entrapment efficiency with passive loading method was low,while the entrapment efficiency was up to 93.27% with active loading method.Conclusion： The sophoridine liposomes with high entrapping efficiency can be prepared by active loading method.

关 键 词：槐定碱 脂质体 主动载药 被动载药 

分 类 号：R283.6[医药卫生—中药学]