大承气汤对内毒素血症大鼠炎性细胞因子的影响  

Effect of Dachengqi Decoction on Cytokines Tumor Necrosis Inflammatory Cytokines in Rats with Endotoxaemia

作　　者：马超英[1] 周鹃[2] 李海霖[2] 耿耘[1] 唐静雯[1] 彭春荣[1] 涂显琴[1] 徐寅生[1] 

机构地区：[1]西南交通大学生命科学与工程学院,成都610031 [2]青海省中医院,西宁810000

出　　处：《中国实验方剂学杂志》2011年第13期130-133,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30873365);中央高校基本科研业务费专项研究项目(SWJTU09ZT29)

摘　　要：目的:观察大承气汤对内毒素血症大鼠炎性细胞因子的影响,探讨大承气汤在治疗内毒素血症中的作用机制。方法:SD大鼠50只,随机分为假手术组、模型组、大承气汤组、大黄组、厚朴组。ig剂量按1g·kg-1给予大鼠,大鼠术前给药2d,末次给药1h后,按盲肠结扎穿孔术(CLP)方法造模,术后连续按相同剂量给药3d,测定大鼠血浆内毒素和血清肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)、白介素-10(IL-10)的含量。结果:与模型组相比,各治疗组血浆内毒素含量、血清TNF-α含量均明显降低(P<0.01);与模型组比较,各治疗组血清IL-1β和IL-10含量均显著降低,差异显著(P<0.01);IL-1β/IL-10模型组(1.218 6±0.3879)、大承气汤组(0.3891±0.2239)、大黄组(0.3667±0.2489)、厚朴组(0.372 5±0.185 8)亦有明显差异(P<0.01)。结论:大承气汤对内毒素血症的作用机制与拮抗血清炎性细胞因子TNF-α,IL-1β和调节IL-1β/IL-10比值有关。Objective：To observe the effect of Dachengqi Decoction（DT）on inflammatory cytokines in rats suffered from the endotoxemia,and to discuss the mechanism of DT when curing the endotoxemia.Method： Fifty SD rats were divided into 5 groups randomly：sham operation group,model group,DT group,Rhei Radix et Rhizome group and Magnolia officinalis group.Two days before model establishment,rats were intervented by intragastric administration（1 g·kg-1）for the medication groups;and 1 hour after the last drug administration,model was established by CLP,then medication was continued for 3 days.The serum content of endotoxin,TNF-α,IL-1β and IL-10 was detected.Result： The content of plasma endotoxin,TNF-α,IL-1β and IL-10 was decreased evidently in curing group compared with those in the model group（P0.01）.Concerning IL-1β/IL-10,model group（1.218 6±0.387 9）,DT group（0.389 1±0.223 9）,Radix Et Rhizome Rhea group（0.366 7±0.248 9）,M.officinalis group（0.372 5±0.185 8）,each treatment group compared with model group also had significant difference（P0.01）.Conclusion： It shows that the mechanism of DT on endotoxemia is related with the antagonistic to the serum inflammatory cytokines TNF-α,IL-1β and the moderating effect to the ratio of IL-1β/IL-10.

关 键 词：内毒素血症 大承气汤 炎性细胞因子 

分 类 号：R285.5[医药卫生—中药学]