全蝎、蜈蚣对胶原诱导型关节炎大鼠肠黏膜免疫状态的调节作用  

Regulation of Scorpio and Scolopendra on Intestinal Mucosal Immunity System in Rats with Collagen Induced Arthritis

作　　者：刘端勇[1] 赵海梅[1] 程绍民 左志琴[1] 吕爱平[3] 

机构地区：[1]江西中医学院科技学院,南昌330025 [2]江西中医学院中诊教研室,南昌330004 [3]中国中医科学院中医临床基础研究所,北京100700

出　　处：《中国实验方剂学杂志》2011年第13期133-136,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30860377);江西省自然科学基金项目(2009GZY0118);江西省卫生厅科技计划(20092050;20092052)

摘　　要：目的:观察全蝎、蜈蚣对胶原蛋白诱导型关节炎(collagen induced arthritis,CIA)大鼠肠黏膜免疫状态的调节作用。方法:Wistar大白鼠60只,随机分成6组,即正常组,模型组,全蝎蜈蚣高,中,低剂量组,Ⅱ型胶原蛋白(type Ⅱ collagen,CII)组。采用Ⅱ型胶原蛋白诱导法复制大鼠关节炎模型,并ig给予全蝎蜈蚣混悬液(0.4,0.2,0.1 g.kg-1)进行干预,CII组予CII蛋白冻干粉60μg.kg-1 ig进行对照,共计40 d。采用流式细胞术分别检测大鼠肠道派伊尔(peyer’s patches,PP)结淋巴细胞亚群水平,采用酶联免疫吸附试验法检测小肠组织匀浆转化生长因子β1(transforming growth factor-β1,TGF-β1)、分泌型免疫球蛋白(secreted immunoglobulin,sIgA)的表达水平。结果:与模型组比较,各治疗组均可明显下调CD4+,CD8+ T细胞水平,升高CD4/CD8水平,并具有显著性意义(P<0.05或P<0.01)。同时,除中剂量组外,其他各治疗组小肠组织匀浆sIgA水平均明显上升(P<0.01),至于TGF-β1,则仅中剂量组和CII组小肠组织匀浆中TGF-β表达明显下降(P<0.05或P<0.01)。结论:全蝎、蜈蚣调节胶原免疫性关节炎黏膜免疫的作用可能通过调节小肠黏膜局部PP结T淋巴细胞亚群平衡,降低肠黏膜TGF-β1和升高sIgA表达水平来实现的。Objective：To observe regulation of scorpio and scolopendra on intestinal mucosal immunity system in rats with collagen induced arthritis（CIA）.Method： Sixty rats were randomly divided into 6 groups：normal control group,model control group,low-dose Scorpio and Scolopendra group,middle-dose Scorpio and Scolopendra group,high-dose Scorpio and Scolopendra group,and typeⅡcollagen group.Rheumatoid arthritis was induced by Collagen.Level of lymphocyte subsets from peyer＇s patches was measured by flow cytometry,and expression of TGF-β1,sIgA in intestine was detected by enzyme linked immunosorbent assay（ELISA）.Result： Cellular level of CD4＋,CD8＋ was obviously descending in all treated groups when compared with the model group（P0.05 or P0.01）.Except for middle-dose Scorpio and Scolopendra group,while expression of sIgA in enteric homogenate from other treated groups was notably heightened（P0.01）.On the contrary,TGF-β1 was remarkably lower in middle-dose Scorpio and Scolopendra group and type Ⅱcollagen group（P0.05 or P0.01）.Conclusion： Regulation of Scorpio and Scolopendra on intestinal mucosal immunity system in rats with collagen induced arthritis was potentially implemented by regulating balance of T-lymphocyte subsets,down-regulating expression of TGF-β1 and increasing sIgA expression in intestine.

关 键 词：类风湿性关节炎 全蝎 蜈蚣 黏膜免疫 

分 类 号：R285.5[医药卫生—中药学]