扶正泄浊保肾汤对腺嘌呤致CRF大鼠肾功能的影响  

Therapeutic Effects of Fuzheng Xiezhuo Baoshen Decoction on Chronic Renal Failure in Rats

作　　者：朱海慧[1] 马居里[1] 严惠芳[1] 迟金亭[1] 张璇[1] 向英歌[1] 

机构地区：[1]陕西中医学院中西医临床医学院,陕西咸阳712045

出　　处：《中国实验方剂学杂志》2011年第13期181-184,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：陕西省教育厅项目(2010JK494)

摘　　要：目的:研究扶正泄浊保肾汤对腺嘌呤致慢性肾功能衰竭(CRF)大鼠的治疗作用及机制。方法:雄性SD大鼠50只随机抽取10只为正常对照组(N),其余40只大鼠采用腺嘌呤ig的方法建立CRF大鼠模型;造模后再将模型大鼠随机分为模型组(M)、氯沙坦钾组(LP,5 mg·kg-1)、扶正泄浊保肾汤低、高剂量组(18.8,37.6 g·kg-1),每组10只;药物治疗30 d后处死大鼠,取标本送检。观察大鼠体重、肾重指数、血生化,并作肾脏病理学检查,检测肾组织转化生长因子-β1(TGF-β1)的表达。结果:扶正泄浊保肾汤2个剂量组大鼠Scr,BUN低于模型组(P<0.01),高剂量组BUN显著低于氯沙坦钾组(P<0.05);扶正泄浊保肾汤2个剂量组肾重指数显著低于模型组(P<0.01)及氯沙坦钾组(P<0.05),高剂量组肾组织TGF-β1的表达明显低于模型组及氯沙坦钾组(P<0.01)。结论:扶正泄浊保肾汤能改善CRF大鼠肾功能,其机制与减少TGF-β1在肾组织的阳性表达,抑制肾小球硬化和肾间质纤维化进展关系密切。Objective：To study the possible mechanism of Fuzheng Xiezhuo Baoshen（FZXZBS）Decoction＇s therapeutic effects on chronic renal failure（CRF） in rats.Method： Ten rats were normal in control group（N）,which were randomly selected from the total 50 male SD rats,the other 40 rats were established chronic renal failure model by gavaging with adenine.And then the model rats were randomly divided into four groups：model group（M）,Losartan Potassium group（LP,5 mg·kg-1）,FZXZBS decoction large and small dose groups（37.6,18.8 g·kg-1）with 10 rats per group.Finally the rats were sacrificed after 30 days treatment.Blood sample and kidney tissue were reserved.The kidney index,blood biochemical indices,pathological changes and expression of transforming growth factor-β1（TGF-β1）in renal tissue were observed.Result：Compared with M,the levels of Scr,BUN in L and S were significantly decreased（P0.01）,and the level of BUN in L was remarkably lower than that in LP（P0.05）.The kidney index in L was obviously lower than those in M and LP（P0.01）,and S was significantly lower than those in M（P0.01）and LP（P0.05）respectively.The expression of TGF-β1 in renal tissue in L was remarkably lower than those in M and LP（P0.01）,and even lower than that in S（P0.05）.Conclusion： FZXZBS Decoction has a preventive and therapeutic effect on CRF.Reducing of the expressions of TGF-β1 in renal tissue may be one of the mechanisms to inhibit the progress of CRF.

关 键 词：扶正泄浊保肾汤 慢性肾功能衰竭 转化生长因子-Β1 肾功能 

分 类 号：R285.5[医药卫生—中药学]