苏云金芽胞杆菌工程菌WG-001可湿性粉剂的增效化合物筛选  

Screening of synergistic inorganic compounds for Bacillus thuringiensis engineering strain WG-001 WP

作　　者：张聪冲[1] 邱浩[1] 覃春华[1,2] 游红[1] 陈火松 李建洪[1] 

机构地区：[1]华中农业大学机物科学技术学院,武汉430070 [2]襄阳市草公司南漳烟叶分公司,襄阳441500 [3]湖北浠水县种子管理局,黄冈438200

出　　处：《植物保护学报》2011年第3期271-275,共5页Journal of Plant Protection

基　　金：公益性行业(农业)科研专项(200803001)

摘　　要：为了筛选出对苏云金芽胞杆菌具有增效作用的无机化合物,并确定其最佳使用浓度,于苏云金芽胞杆菌工程菌WG-001可湿性粉剂(B tWG-001 WP)中添加MgC l2、CaC l2、NaHCO3、MgSO4、NaH2PO4、Na2CO3、Na2HPO4、KH2PO4等8种化合物,测定其对小菜蛾的毒力。结果显示,添加0.4%MgC l2、0.2%CaC l2、0.4%MgSO4、0.2%Na2CO3时,共毒因子分别为4 6.7 5、3 6.2 7、41.51、31.03,均大于20,而添加另外4种化合物,共毒因子均低于20;添加0.4%MgC l2、0.2%CaC l2、0.4%MgSO4、0.2%Na2CO3,增效比最大,分别为1.46、1.33、1.34、1.29。田间试验结果显示,BtWG-001 WP中添加0.4%MgSO4稀释500倍施用时,药后7天对小菜蛾的田间平均防效最高,为80.51%,且与其它处理差异显著。表明添加0.4%MgSO4可显著提高B t对小菜蛾的田间防效。To screen synergistic and optimal application concentration of inorganic compounds for Bacillus thuringiensis engineering strain WG-001 against diamondback moth,Plutella xylostella,toxicity of Bt WG-001 WP to P.xylostella containing inorganic compounds,such as MgCl2,CaCl2,NaHCO3,MgSO4,NaH2PO4,Na2CO3,Na2HPO4,KH2PO4,was evaluated.Results showed that co-toxicity factors of 0.4%MgCl2,0.2% CaCl2,0.4%MgSO4 and 0.2%Na2CO3 with Bt WG-001 WP were 46.75,36.27,41.51 and 31.03,respectively.While the other 4 kinds of inorganic compounds had no synergetic action to Bt WG-001 WP against P.xylostella.Synergistic ratios of 0.4%MgCl2,0.2%CaCl2,0.4%MgSO4 and 0.2%Na2CO3 with Bt WG-001 WP were 1.46,1.33,1.34 and 1.29 respectively.Results of field test showed that average control effect of 500 dilution of Bt WG-001 WP containing 0.4%MgSO4 against P.xylotella was 80.51% on the 7th day after spraying,and it had significantly difference from other treatments.The result indicated that 0.4% MgSO4 could significantly increase field control efficacy of Bt WG-001 WP against P.xylotella.

关 键 词：小菜蛾 苏云金芽胞杆菌工程菌WG-001 无机化合物 增效作用 防治效果 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]