甜菜碱合成酶CMO、BADH基因无抗生素标记植物表达载体的构建  

Construction of Non-antibiotic Maker Plant Expression Vector on Choline Monooxygenase and Betaine Aldehyde Dehydrogenase Gene

作　　者：邓川[1] 高翎[1] 张君[1] 宋冰[1,2] 王丕武[1] 

机构地区：[1]吉林农业大学生物技术中心,吉林长春130118 [2]吉林市农业科学院,吉林吉林132101

出　　处：《种子》2011年第6期13-15,18,共4页Seed

基　　金：教育部博士点基金(编号:20070193005)

摘　　要：利用基因工程技术,对植物表达载体进行改造,去除NptⅡ、Hyg等抗生素标记,获得安全载体骨架,分别构建了无抗生素选择标记的CMO、BADH以及双价基因的植物表达载体pROK-CMO、pCAMBIA-1301-BADH1、pCAMBIA-1301-CMO+BADH。通过冻融法将其转入根癌农杆菌LBA4404中得到工程菌株,为下一步安全转化奠定基础。By the genetic engineering technology, plant expression vectors were reconstructed to form a safe carrier skeleton,which were knocked out the antibiotic markers genes,such as NptII and Hyg. The Non-antibiotic maker Plant Expression Vectors with CMO or BADH gene were constructed respectively. Together with dual price of the plant expression vectors such as pROK-CMO, pCAMBIA-1301-BADH 1, pCAMBIA-1301- CMO ＋ BADH were constructed later. The freeze-thaw method was used to transfer these vectors into the agrobacterium strain LBA 4404 in order to acquire the engineering bacteria and laid the foundation for the security transformation of the next step.

关 键 词：甜菜碱 CMO BADH 无抗生素标记 植物表达载体 

分 类 号：Q782[生物学—分子生物学]