34株多重耐药铜绿假单胞菌β内酰胺酶基因及Ⅰ类整合子检测分析  

Analysis of the resistance gene of beta-lactamase and classⅠintegron in 34 strains of multidrug-resistant Pseudomonas aeruginosa

作　　者：万瑛[1] 阴晴[1] 成静[2] 段秀杰[1] 高雁[1] 陆建成[1] 

机构地区：[1]江苏大学附属医院检验科,江苏镇江212001 [2]江苏大学基础医学与技术学院,江苏镇江212013

出　　处：《海南医学院学报》2011年第7期886-889,共4页Journal of Hainan Medical University

基　　金：江苏大学临床医学科技发展基金资助(JLY2010166)~~

摘　　要：目的:探讨我院临床分离多重耐药铜绿假单胞菌的β-内酰胺酶相关基因及Ⅰ类整合子(intⅠ1和qacE△1-sul1)的携带情况,为临床抗感染治疗和控制医院感染提供依据。方法:采用K-B法对临床分离的铜绿假单胞菌进行药敏试验,选出34株多重耐药菌株,用PCR法检测其相关耐药基因16种。结果:在34株多重耐药铜绿假单胞菌中共有13种耐药基因阳性,阳性率较高的为qacE△1-sul1、oprD2缺失、blaTEM。结论:多重耐药铜绿假单胞菌可同时获得多种耐药基因,临床应根据药敏结果选择用药,以减少耐药菌株的产生,控制院内感染。Objective： To explore the resistance gene and prevalence of beta-lactamas and classⅠintegron of clinical isolates of multidrug-resistant Pseudomonas aeruginosa（MDRPA） in our hospital,and provide evidence for clinical anti-infective therapy and control the nosocomial infection.Methods： Antimicrobial susceptibilities of the Pseudomonas aeruginosa isolates were detected by Kirby Bauer method.Out of 34 collected MDRPA strains,16 kinds of resistance genes were detected by polymerase chain reaction（PCR） method.Results： Thirteen kinds of resistant genes were detected as positive for the 34 strains.Relevant higher positive rates were observed for qacE△1-sul1、oprD2 defect and blaTEM.Conclusions： Multidrug-resistant Pseudomonas aeruginosa have several kinds of resistant genes.Antibiotics should be used rationally according to antimicrobial susceptibility in order to control the development of multidrug bacteria and nosocomial infection.

关 键 词：铜绿假单胞菌 耐药基因 Β-内酰胺酶 Ⅰ类整合子 

分 类 号：R446.5[医药卫生—诊断学]