不同血液稀释方法对直肠癌患者围术期NK细胞和CIK细胞的影响  

Effects of different hemodilution techniques on perioperative NK and CIK cells in patients undergoing rectal cancer surgery

作　　者：戴春宇[1] 赵光瑜[1] 吕志勇[1] 

机构地区：[1]昆明医学院第三附属医院麻醉科,昆明市650031

出　　处：《临床麻醉学杂志》2011年第6期563-565,共3页Journal of Clinical Anesthesiology

基　　金：云南省教育厅科学研究基金项目(项目编号:06y140c)

摘　　要：目的比较急性非等容血液稀释（ANIH）和急性高容血液稀释（AHH）联合控制性降压（CH）对直肠癌患者围术期自然杀伤（NK）细胞和细胞因子诱导杀伤（CIK）细胞的影响。方法择期直肠癌根治术患者45例，随机均分为ANIH组、AHH＋CH组和对照组。分别于麻醉前1h（T1）、术毕即刻（T2）、术后第1天（T3）、第4天（T4）抽取静脉血检测NK和CIK细胞的数量。结果与T1时比较，T2～T4时AHH＋CH组NK细胞明显升高（P〈0．05），T4时ANIH组NK细胞明显升高（P〈0．05），而对照组则明显降低（P〈0．05）。与T1时比较，T2时三组CIK细胞均明显降低（P〈0．05），T4时ANIH组和AHH＋CH组均明显升高（P〈0．05），且明显高于对照组（P〈0．05）。结论AHH联合CH以及ANIH均能使直肠癌患者NK和CIK细胞增加，AHH联合CH效果更优。Objective To evaluate the effects of acute non-normolvolemic hemodilution（ANIH） and acute hypervolemic hemodilution（AHH）combined with controlled hypotension（CH）on NK and CIK cells in patients undergoing rectal cancer. Methods Forty-five patients undergoing elective rectal cancer surgery were randomly divided into three groups （n= 15）： group ANIH, group AHH＋CH and control group. The venous blood samples were taken at 1 h before anesthesia （T1）, at the end of the surgery （T2）, lday （T3） and the 4 day （T4） after surgery to measure the percentage of NK and CIK cells. Results The percentage of NK cells in AHH＋CH group were significantly increased at T2 ,T3and T4 （P〈0.05）, and it also obviously increased in ANIH group at T4 （P〈0.05）, but it obviously decreased in control group at T4 （P〈0.05）. Compared with T1 ,CIK cells in all three groups obviously decreased at T2 （P〈0.05） ; the percentage of CIK cells in ANIH and AHH＋CH group were significantly increased at T4 （P〈0.05）. Conclusion AHH＋CH and ANIH can increase the percentage of NK and CIK cells in patients undergoing rectal cancer, AHH combined CH even better.

关 键 词：直肠癌 血液稀释 自然杀伤细胞 细胞因子诱导杀伤细胞 

分 类 号：R614[医药卫生—麻醉学]