局部温热对接触性超敏反应小鼠皮肤朗格汉斯细胞的影响  

Effect of local hyperthermia on Langerhans cells in the skin of a mouse model of contact hypersensitivity

作　　者：王一如[1] 陆东庆[1] 张岚[2] 张丽[2] 王雅坤[2] 李晓东[2,3] 洪玉晓[2] 刘哲[2] 高兴华[2] 

机构地区：[1]中山大学附属第五医院皮肤科,珠海519000 [2]中国医科大学附属第一医院皮肤科 [3]沈阳医学院奉天医院皮肤科

出　　处：《中华皮肤科杂志》2011年第7期468-471,共4页Chinese Journal of Dermatology

基　　金：国家自然科学基金（30972659）

摘　　要：目的探讨局部温热对小鼠接触性超敏反应激发部位朗格汉斯细胞（LC）数目和形态的影响。方法8周龄雌性SPF级Balb／c小鼠60只，随机分为致敏前3d加热组、致敏同时加热组和致敏2d后加热组，每组20只。分别于致敏前3d、致敏同时和致敏2d后在背部致敏部位施以37℃、39℃、41℃、43℃的局部温热（各5只）20min。同时设立5只致敏一不加热组作为对照。于小鼠右耳背侧激发后2d，分离右耳背侧表皮，采用免疫组化方法检测表皮Lc的数目和形态变化。结果随所施温度的升高，LC数目在致敏前加热组逐渐减少，37℃、39℃、41℃、43qC依次为（321．83±41．81）、（251．12±16．29）、（191．41±28．7）、（128．33±77．61）个／mm^2，组间差异有统计学意义（P〈0．05）；在致敏同时加热组逐渐增多，但差异无统计学意义（P〉0．05）；在致敏后加热组也逐渐增多，依次为（320．83±113．6）、（398．33±31．91）、（437．83±29．78）、（477．25±86．79）个／mm2,差异有统计学意义（P〈0．01）。无论致敏前3d、致敏同时和致敏2d后施以不同温热条件，LC的树突随所施温度升高呈增多、增长的趋势；但在43℃条件下，其趋势略有下降。结论致敏部位的局部温热对激发部位LC形态和密度有影响，可能与接触性超敏反应的严重程度有关。Objective To investigate the effect of local hyperthermia on the morphology and quantity of Langerhans cells （LCs） at challenged skin sites of a mouse model of contact hypersensitivity. Methods Sixty mice were equaUy divided into 3 groups to be treated with local hyperthermia （37 ℃, 39 ℃, 41 ℃ and 43 ℃） for 20 minutes at sensitization sites on the back of mice 3 days before （pre-heat group）, concurrently with （concurrent-heat group） or 2 days after （post-heat group） sensitization respectively. Five mice which remained unsensitized and untreated served as the controls. Five days after the sensitization, the mice were challenged on the dorsal surface of right ears. Two days after the elicitation, the right ears were resected and immunohistochemistry was performed to observe the morphology and determine the quantity of LCs at challenged sites. Results With the rise in temperature, the number of LCs in the epidermis of ear skin decreased in pre-heat group （321.83 ± 41.81,251.12± 16.29, 191.41 ± 28.7, 128.33 ＋ 77.61 per square millimeter at 37 ℃, 39 ℃, 41 ℃ and 43 ℃, respectively, P 〈 0.05）, but increased gradually in the concurrent-heat group （309.08 ±84.69, 355.33± 11.38, 405.5 ±55.25, 438.16 ±99.56 per square millimeter at 37℃, 39℃, 41 ℃ and 43 ℃, respectively, P 〉 0.05） and in the post-heat group （320.83 ± 113.6, 398.33 ± 31.91,437.83 ± 29.78, 477.25 ±86.79 per square millimeter at 37 ℃, 39 ℃, 41 ℃ and 43℃, respectively, P 〈 0.01 ）. The dendrites of LCs increased in number and length when the temperature rose from 37 ℃ to 41 ℃, but slightly declined at 43 ℃. Conclusions Local hyperthermia at sensitization sites could affect the morphology and density of LCs at challenged sites, and the effect is likely associated with the severity of contact hypersensitivity.

关 键 词：皮炎 接触性 郎格尔汉斯细胞 高温 诱发 疾病模型 动物 

分 类 号：R392.1[医药卫生—免疫学]