遮光剂对小鼠迟发型变态反应的影响  

Effects of sunscreens on delayed type hypersensitivity in mice

作　　者：朱慧兰[1] 赵晓霞[1] 李润祥[1] 梁碧华[1] 黄茂芳[1] 罗育武[1] 马少吟[1] 张锡宝[1] 

机构地区：[1]广州市皮肤病防治所,510095

出　　处：《中华皮肤科杂志》2011年第7期505-508,共4页Chinese Journal of Dermatology

基　　金：2008年广东省自然科学基金（8151009503000001）;2008年和2009年广州市医药卫生科技项目（2008-YB-229,2009-YB-235）

摘　　要：目的探讨不同防晒效能遮光剂对小鼠迟发型变态反应的影响和在小鼠免疫保护中的作用。方法将Balb／c小鼠随机分成5组，每组10只，分别为Ⅰ组：阳性对照组，不予照射；Ⅱ组：单纯模拟太阳辐射（SSR）组；Ⅲ组：遮光剂1组（SPF15，PPD12）；Ⅳ组：遮光剂2组（SPF50，PPD28）；V组：阴性对照组。Ⅱ～V组均予SSR照射10d，剂量为1．4J／cm^2UVA＋100mJ／cm^2UVB，照射第6天时，Ⅰ、Ⅱ、Ⅲ、Ⅳ组腹腔注射白念珠菌菌丝，并于第10天照射结束后测量所有小鼠双后足足垫，并于足垫内注射白念珠菌菌丝，24h后测量其厚度，计算出免疫抑制率。Western印迹检测小鼠皮肤中CDla、CD80、CD86的表达，比较其差异。结果阳性对照组、遮光剂1组和遮光剂2组小鼠的足垫水肿厚度（分别为0．41±0．38、0．21±0．23、0．30±0．25mm）明显高于阴性对照组（0．04±-O．03mm）及SSR组（0．14±0．12mm），P值均〈0．05，但两种遮光剂组之间差异无统计学意义（P〉0．05）。各组免疫抑制率SSR组为73．0％±11．3％，遮光剂1组为54．1％±6．4％，遮光剂2组为29．7％±7．5％，组间差异有统计学意义（P〈0．01）。Western印迹检测，阳性对照组小鼠皮肤中CDla蛋白含量明显高于SSR组，阳性对照组和遮光剂1组的CD86均高于SSR组和阴性对照组（P〈0．05），其余各组CDla、CD80、CD86表达差异无统计学意义（P〉0．05）。结论亚红斑量紫外线可诱发迟发型变态反应，遮光剂有免疫保护作用，朗格汉斯细胞在紫外线诱导的免疫抑制作用中不是必需的。Objective To study the influence of sunscreens with different efficacy on delayed type hypersensitivity （DTH） and their immunoprotective effect in mice. Methods A cohort of mice were randomly divided into 5 groups with 10 mice in each group： group 1 as the positive control without irradiation, group 2 receiving solar-simulated radiation （SSR） only, group 3 receiving SSR and protected by sunsereen 1 with sun protection factor 15 （SPF15） and persistent pigment darkening （PPD） 12, group 4 receiving SSR and protected by sunscreen 2 with SPF 50 and PPD 28, and group 5 as the negative control receiving SSR only. SSR was carried out on the back of mice with the UVA dose being 1.4 J/cm^2 and UVB dose being 100 mJ/cm^2 for 10 days. After a 5-day irradiation, the groups 1 to 4 were immunized by intraperitoneal injection with 100 μl （10^7 cells/ml） of Candida albicans suspension. On the 10^th day both sides of the posterior foot pad were measured; then the foot pads were injected with additional 50μl of the Candida albieans suspension. Twenty-four hours after the injection, the thickness of each foot pad was measured, and immunosuppression rate was calculated. Finally, the mice were sacrificed and skin samples were obtained from the back of these mice followed by the examination of CDla, CD80 and CD86 expression by Western blot. Results The thickness of edema in foot pads was 0.41±0.38 mm, 0.21±0.23 mm and 0.30± 0.25 mm in group 1, 3 and 4, respectively, significantly higher than in group 5 and 2 （0.04 ± 0.03 ram, 0.14 ± 0.12 mm, respectively, all P 〈 0.05）, while no significant difference was observed between the group 3 and 4 （P 〉 0.05）. Significant differences were observed in the immunosup- pression rate between group 2, 3 and 4 （73.0% ±11.3%, 54.1% ± 6.4%, 29.7%±7.5%, respectively, all P〈 0.01）. Western blot revealed a significant increment in the expression of CDla protein in group 1 compared with group 2 as well as in the expression of CD86 protein in group 1 and grou

关 键 词：紫外线 防晒剂 皮炎 光变态反应 郎格尔汉斯细胞 疾病模型 动物 

分 类 号：R751[医药卫生—皮肤病学与性病学]