CO_2激光对豚鼠耳蜗结构影响的实验研究  

Experimental study of the effect that CO_2 laser on morphology of guinea pig cochlea

作　　者：李栋[1] 叶青[1] 耿阳[1] 田天捷[1] 赵海滨[2] 詹振林[2] 张先增[2] 

机构地区：[1]福建医科大学省立临床医学院耳鼻咽喉科,福州350001 [2]福建师范大学医学光电科学与技术教育部重点实验室

出　　处：《福建医药杂志》2011年第3期1-2,108,共3页Fujian Medical Journal

基　　金：国家自然科学基金资助项目(60878062);卫生部科学研究基金福建省卫生教育联合攻关计划项目(WKJ2008-2-35)

摘　　要：目的研究低峰功率超脉冲CO2激光对豚鼠耳蜗结构的影响。方法将24只豚鼠随机分为激光2W组、激光4 W组及对照组,每组8只,前两组分别以超脉冲CO2激光功率2 W、4 W行左耳蜗底周造孔,对照组只手术暴露耳蜗底周。术后21 d处死动物,应用耳蜗基底膜铺片、扫描电镜技术观察豚鼠耳蜗形态学的改变。结果实验组与对照组的豚鼠耳蜗在形态学上基本一致,结构改变不明显,其中激光2 W组外毛细胞纤毛稍散乱,无缺失;激光4 W组表现为外毛细胞纤毛散乱,以第3排为著,并有纤毛散在缺失。结论低峰功率超脉冲CO2激光(峰功率为125 W)能够很好的消融豚鼠耳蜗底周骨壁,且随功率增大消融效果更明显,但对耳蜗的结构影响轻微。Objective Experiment in guinea pigs were performed to study the effect of superpulsed CO2 laser irradiation in different power on the cochlea.Methods Low peak power of superpulsed CO2 laser was adopted,24 guinea pigs were divided into the laser 2 W group,laser 4 W group and the control group,with eight in each group.The former two groups were performed fenestration on the basal turn of cochlea using laser irradiation with power 2 W and 4 W respectively,the Control group only exposed basal turn of cochlea.Light and scanning electron microscope were performed to observe the changes of cochlear morphology.Results The morphology of the laser 2 W group changed slightly,and laser 4 W group showed hair cell stereocilia scattered mainly and missing less,compared with the control group which was usually normal.Conclusion The experimental results presented indicated that low peak power superpulsed CO2 laser can ablate the bone tissue of cochlea efficiently,and had a high application safety for laser fenestration in guinea pigs.

关 键 词：CO2激光 耳蜗 损伤 

分 类 号：R764.35[医药卫生—耳鼻咽喉科]